Technical Resources - Media Formulations

11330 - DMEM/F-12, HEPES

Catalog Number(s):
11330032 ,11330057 ,11330099 ,11330107

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 18.75 0.25
L-Alanine 89 4.45 0.05
L-Arginine hydrochloride 211 147.5 0.699
L-Asparagine-H2O 150 7.5 0.05
L-Aspartic acid 133 6.65 0.05
L-Cysteine hydrochloride-H2O 176 17.56 0.0998
L-Cystine 2HCl 313 31.29 0.1
L-Glutamic Acid 147 7.35 0.05
L-Glutamine 146 365 2.5
L-Histidine hydrochloride-H2O 210 31.48 0.15
L-Isoleucine 131 54.47 0.416
L-Leucine 131 59.05 0.451
L-Lysine hydrochloride 183 91.25 0.499
L-Methionine 149 17.24 0.116
L-Phenylalanine 165 35.48 0.215
L-Proline 115 17.25 0.15
L-Serine 105 26.25 0.25
L-Threonine 119 53.45 0.449
L-Tryptophan 204 9.02 0.0442
L-Tyrosine disodium salt dihydrate 261 55.79 0.214
L-Valine 117 52.85 0.452
Vitamins
Biotin 244 0.0035 0.0000143
Choline chloride 140 8.98 0.0641
D-Calcium pantothenate 477 2.24 0.0047
Folic Acid 441 2.65 0.00601
Niacinamide 122 2.02 0.0166
Pyridoxine hydrochloride 206 2 0.00971
Riboflavin 376 0.219 0.000582
Thiamine hydrochloride 337 2.17 0.00644
Vitamin B12 1355 0.68 0.000502
i-Inositol 180 12.6 0.07
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 116.6 1.05
Cupric sulfate (CuSO4-5H2O) 250 0.0013 0.0000052
Ferric Nitrate (Fe(NO3)3"9H2O) 404 0.05 0.000124
Ferric sulfate (FeSO4-7H2O) 278 0.417 0.0015
Magnesium Chloride (anhydrous) 95 28.64 0.301
Magnesium Sulfate (MgSO4) (anhyd.) 120 48.84 0.407
Potassium Chloride (KCl) 75 311.8 4.16
Sodium Bicarbonate (NaHCO3) 84 1200 14.29
Sodium Chloride (NaCl) 58 6995.5 120.61
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 71.02 0.5
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 62.5 0.453
Zinc sulfate (ZnSO4-7H2O) 288 0.432 0.0015
Other Components
D-Glucose (Dextrose) 180 3151 17.51
HEPES 238 3574.5 15.02
Hypoxanthine Na 159 2.39 0.015
Linoleic Acid 280 0.042 0.00015
Lipoic Acid 206 0.105 0.00051
Phenol Red 376.4 8.1 0.0215
Putrescine 2HCl 161 0.081 0.000503
Sodium Pyruvate 110 55 0.5
Thymidine 242 0.365 0.00151

Reference:

  1. Dulbecco, R. and Freeman, G., (1959) Plaque formation by the polyoma virus. Virology 8:396.
  2. Ham, R.G., (1965) Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
  3. Morton, H.J. (1970) A survey of commercially available tissue culture media. In Vitro 6(2):89.
  4. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185.
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