Technical Resources - Media Formulations
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 50 | 0.667 |
| L-Alanine | 89 | 90 | 1.01 |
| L-Arginine | 174 | 50 | 0.287 |
| L-Asparagine-H2O | 150 | 20 | 0.133 |
| L-Aspartic acid | 133 | 30 | 0.226 |
| L-Cysteine | 121 | 40 | 0.331 |
| L-Cystine 2HCl | 313 | 26.07 | 0.0833 |
| L-Glutamic Acid | 147 | 50 | 0.34 |
| L-Histidine | 155 | 15 | 0.0968 |
| L-Isoleucine | 131 | 50 | 0.382 |
| L-Leucine | 131 | 75 | 0.573 |
| L-Lysine hydrochloride | 183 | 87.46 | 0.478 |
| L-Methionine | 149 | 15 | 0.101 |
| L-Phenylalanine | 165 | 25 | 0.152 |
| L-Proline | 115 | 30 | 0.261 |
| L-Serine | 105 | 10 | 0.0952 |
| L-Threonine | 119 | 40 | 0.336 |
| L-Tryptophan | 204 | 10 | 0.049 |
| L-Tyrosine disodium salt dihydrate | 261 | 50.65 | 0.194 |
| L-Valine | 117 | 50 | 0.427 |
| Vitamins | |||
| Ascorbic Acid | 176 | 2 | 0.0114 |
| Biotin | 244 | 0.5 | 0.00205 |
| Choline chloride | 140 | 1.5 | 0.0107 |
| D-Calcium pantothenate | 477 | 1 | 0.0021 |
| Ergocalciferol | 397 | 0.1 | 0.000252 |
| Folic Acid | 441 | 1 | 0.00227 |
| Menadione sodium bisulfate | 276 | 0.01 | 0.0000362 |
| Niacinamide | 122 | 1 | 0.0082 |
| Pyridoxal hydrochloride | 204 | 1 | 0.0049 |
| Riboflavin | 376 | 0.1 | 0.000266 |
| Thiamine hydrochloride | 337 | 1 | 0.00297 |
| Vitamin A (acetate) | 328 | 0.1 | 0.000305 |
| Vitamin B12 | 1355 | 0.2 | 0.000148 |
| alpha Tocopherol acetate Na | 702 | 0.01 | 0.0000142 |
| i-Inositol | 180 | 2 | 0.0111 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 200 | 1.8 |
| Cupric sulfate (CuSO4-5H2O) | 250 | 0.0001 | 0.0000004 |
| Ferric sulfate (FeSO4-7H2O) | 278 | 0.0001 | 0.0000004 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 97.67 | 0.814 |
| Manganese Sulfate (MnSO4-H20) | 169 | 0.0001 | 0.0000006 |
| Potassium Chloride (KCl) | 75 | 400 | 5.33 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2200 | 26.19 |
| Sodium Chloride (NaCl) | 58 | 6800 | 117.24 |
| Sodium Phosphate monobasic (NaH2PO4) anhydrous | 138 | 140 | 1.01 |
| Zinc sulfate (ZnSO4-7H2O) | 288 | 0.0002 | 0.0000007 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 2000 | 11.11 |
| Glutathione (reduced) | 307 | 0.05 | 0.000163 |
| Methyl linoleate | 295 | 0.03 | 0.000102 |
| Phenol Red | 376.4 | 10 | 0.0266 |
| Sodium Pyruvate | 110 | 25 | 0.227 |
Reference:
- Williams, G.M. and Gunn, J.M. (1974) Long term cell culture of adult rat liver epithelial cells. Exp. Cell Res., 89:139.
