Technical Resources - Media Formulations
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 50 | 0.667 |
| L-Arginine hydrochloride | 211 | 75 | 0.355 |
| L-Aspartic acid | 133 | 60 | 0.451 |
| L-Cysteine | 121 | 61 | 0.504 |
| L-Cystine | 240 | 15 | 0.0625 |
| L-Glutamic Acid | 147 | 150 | 1.02 |
| L-Glutamine | 146 | 350 | 2.4 |
| L-Histidine | 155 | 128 | 0.826 |
| L-Isoleucine | 131 | 25 | 0.191 |
| L-Leucine | 131 | 50 | 0.382 |
| L-Lysine hydrochloride | 183 | 240 | 1.31 |
| L-Methionine | 149 | 50 | 0.336 |
| L-Phenylalanine | 165 | 50 | 0.303 |
| L-Proline | 115 | 50 | 0.435 |
| L-Threonine | 119 | 75 | 0.63 |
| L-Tryptophan | 204 | 40 | 0.196 |
| L-Tyrosine | 181 | 40 | 0.221 |
| L-Valine | 117 | 65 | 0.556 |
| Vitamins | |||
| Ascorbic Acid | 176 | 17.5 | 0.0994 |
| Biotin | 244 | 0.02 | 0.000082 |
| Choline chloride | 140 | 250 | 1.79 |
| D-Calcium pantothenate | 477 | 1 | 0.0021 |
| Folic Acid | 441 | 0.5 | 0.00113 |
| Niacinamide | 122 | 1 | 0.0082 |
| Pyridoxine hydrochloride | 206 | 1 | 0.00485 |
| Riboflavin | 376 | 1 | 0.00266 |
| Thiamine hydrochloride | 337 | 10 | 0.0297 |
| Vitamin B12 | 1355 | 0.2 | 0.000148 |
| i-Inositol | 180 | 1 | 0.00556 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2-2H2O) | 147 | 120 | 0.816 |
| Magnesium Chloride (MgCl2-6H2O) | 203 | 240 | 1.18 |
| Magnesium Sulfate (MgSO4-7H2O) | 246 | 200 | 0.813 |
| Potassium Chloride (KCl) | 75 | 150 | 2 |
| Potassium Phosphate monobasic (KH2PO4) | 136 | 80 | 0.588 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2240 | 26.67 |
| Sodium Chloride (NaCl) | 58 | 6000 | 103.45 |
| Sodium Phosphate dibasic (Na2HPO4-7H2O) | 268 | 566 | 2.11 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 5000 | 27.78 |
| Glutathione (reduced) | 307 | 15 | 0.0489 |
| Hypoxanthine | 136 | 25 | 0.184 |
| Phenol Red | 376.4 | 10 | 0.0266 |
Reference:
- Waymouth, C. (1968) personal communication.
