Technical Resources - Media Formulations
21475 - Fischer’s Medium
Catalog Number(s):
21475025
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| L-Arginine hydrochloride | 211 | 18.6 | 0.0882 |
| L-Asparagine | 132 | 10 | 0.0758 |
| L-Cystine 2HCl | 313 | 26.2 | 0.0837 |
| L-Glutamine | 146 | 204 | 1.4 |
| L-Histidine hydrochloride-H2O | 210 | 66 | 0.314 |
| L-Isoleucine | 131 | 75 | 0.573 |
| L-Leucine | 131 | 30 | 0.229 |
| L-Lysine hydrochloride | 183 | 50 | 0.273 |
| L-Methionine | 149 | 100 | 0.671 |
| L-Phenylalanine | 165 | 60 | 0.364 |
| L-Serine | 105 | 15 | 0.143 |
| L-Threonine | 119 | 30 | 0.252 |
| L-Tryptophan | 204 | 10 | 0.049 |
| L-Tyrosine disodium salt dihydrate | 261 | 86.5 | 0.331 |
| L-Valine | 117 | 70 | 0.598 |
| L-Valine | 117 | 70 | 0.598 |
| Vitamins | |||
| Choline chloride | 140 | 1.5 | 0.0107 |
| D-Calcium pantothenate | 477 | 0.5 | 0.00105 |
| Folic Acid | 441 | 10 | 0.0227 |
| Niacinamide | 122 | 0.5 | 0.0041 |
| Pyridoxal hydrochloride | 204 | 0.5 | 0.00245 |
| Riboflavin | 376 | 0.5 | 0.00133 |
| Thiamine hydrochloride | 337 | 1 | 0.00297 |
| d-Biotin | 244 | 0.01 | 0.000041 |
| i-Inositol | 180 | 1.5 | 0.00833 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 68.7 | 0.619 |
| Magnesium Chloride (anhydrous) | 95 | 47 | 0.495 |
| Potassium Chloride (KCl) | 75 | 400 | 5.33 |
| Sodium Bicarbonate (NaHCO3) | 84 | 1125 | 13.39 |
| Sodium Chloride (NaCl) | 58 | 8000 | 137.93 |
| Sodium Phosphate dibasic (Na2HPO4) anhydrous | 268 | 59.9 | 0.224 |
| Sodium Phosphate monobasic (NaH2PO4-H2O) | 138 | 69 | 0.5 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 1000 | 5.56 |
| Phenol Red | 376.4 | 5 | 0.0133 |
