Technical Resources - Media Formulations
21180 - Medium 199, 10x
Catalog Number(s):
21180021
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 500 | 6.67 |
| L-Alanine | 89 | 250 | 2.81 |
| L-Arginine hydrochloride | 211 | 700 | 3.32 |
| L-Aspartic acid | 133 | 300 | 2.26 |
| L-Cysteine hydrochloride-H2O | 176 | 1.1 | 0.00625 |
| L-Cystine 2HCl | 313 | 260 | 0.831 |
| L-Glutamic Acid-H2O | 165 | 750 | 4.55 |
| L-Glutamine | 146 | 1000 | 6.85 |
| L-Histidine hydrochloride-H2O | 210 | 218.8 | 1.04 |
| L-Hydroxyproline | 131 | 100 | 0.763 |
| L-Isoleucine | 131 | 400 | 3.05 |
| L-Leucine | 131 | 600 | 4.58 |
| L-Lysine hydrochloride | 183 | 700 | 3.83 |
| L-Methionine | 149 | 150 | 1.01 |
| L-Phenylalanine | 165 | 250 | 1.52 |
| L-Proline | 115 | 400 | 3.48 |
| L-Serine | 105 | 250 | 2.38 |
| L-Threonine | 119 | 300 | 2.52 |
| L-Tryptophan | 204 | 100 | 0.49 |
| L-Tyrosine disodium salt dihydrate | 261 | 580 | 2.22 |
| L-Valine | 117 | 250 | 2.14 |
| Vitamins | |||
| Alpha-tocopherol Phosphate | 702 | 0.1 | 0.000142 |
| Ascorbic Acid | 176 | 0.5 | 0.00284 |
| Biotin | 244 | 0.1 | 0.00041 |
| Choline chloride | 140 | 5 | 0.0357 |
| D-Calcium pantothenate | 477 | 0.1 | 0.00021 |
| Folic Acid | 441 | 0.1 | 0.000227 |
| Menadione (Vitamin K3) | 172 | 0.1 | 0.000581 |
| Niacinamide | 122 | 0.25 | 0.00205 |
| Nicotinic acid (Niacin) | 123 | 0.25 | 0.00203 |
| Para-Aminobenzoic Acid | 137 | 0.5 | 0.00365 |
| Pyridoxal hydrochloride | 204 | 0.25 | 0.00123 |
| Pyridoxine hydrochloride | 206 | 0.25 | 0.00121 |
| Riboflavin | 376 | 0.1 | 0.000266 |
| Thiamine hydrochloride | 337 | 0.1 | 0.000297 |
| Vitamin A (acetate) | 328 | 1 | 0.00305 |
| Vitamin D2 (Calciferol) | 397 | 1 | 0.00252 |
| i-Inositol | 180 | 0.5 | 0.00278 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 2000 | 18.02 |
| Ferric nitrate (Fe(NO3)-9H2O) | 404 | 7.2 | 0.0178 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 970 | 8.08 |
| Potassium Chloride (KCl) | 75 | 4000 | 53.33 |
| Sodium Chloride (NaCl) | 58 | 68000 | 1172.41 |
| Sodium Phosphate monobasic (NaH2PO4) anhydrous | 138 | 1400 | 10.14 |
| Other Components | |||
| 2-deoxy-D-ribose | 134 | 5 | 0.0373 |
| Adenine sulfate | 404 | 100 | 0.248 |
| Adenosine 5'-phosphate | 347 | 2 | 0.00576 |
| Adenosine 5'-triphosphate | 605 | 10 | 0.0165 |
| Cholesterol | 387 | 2 | 0.00517 |
| D-Glucose (Dextrose) | 180 | 10000 | 55.56 |
| Glutathione (reduced) | 307 | 0.5 | 0.00163 |
| Guanine hydrochloride | 188 | 3 | 0.016 |
| Hypoxanthine Na | 158 | 3.5 | 0.0222 |
| Phenol Red | 376.4 | 200 | 0.531 |
| Ribose | 150 | 5 | 0.0333 |
| Sodium Acetate | 82 | 500 | 6.1 |
| Thymine | 126 | 3 | 0.0238 |
| Tween 80® | 200 | ∞ | |
| Uracil | 112 | 3 | 0.0268 |
| Xanthine-Na | 152 | 3.44 | 0.0226 |
Reference:
- Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.
- Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. Biol. Med., 73:1.
