Technical Resources - Media Formulations
Medium 254
Without Calcium.
A sterile, liquid medium for the culture of human epidermal melanocytes. Medium 254CF is Medium 254 prepared without calcium chloride. Calcium chloride is provided as a separate component with each bottle of medium. This basal medium requires the addition of HMGS (S-002-5) or HMGS-2 (S-016-5) prior to use. Below is the composition list. The concentrations are proprietary.
Catalog Number(s):
M254CF500
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 0 | 0 |
| L-Alanine | 89 | 0 | 0 |
| L-Alanyl-L-Glutamine | 217 | 0 | 0 |
| L-Arginine hydrochloride | 211 | 0 | 0 |
| L-Asparagine-H2O | 150 | 0 | 0 |
| L-Aspartic acid | 133 | 0 | 0 |
| L-Cysteine | 121 | 0 | 0 |
| L-Glutamic Acid | 147 | 0 | 0 |
| L-Histidine hydrochloride-H2O | 210 | 0 | 0 |
| L-Isoleucine | 131 | 0 | 0 |
| L-Leucine | 131 | 0 | 0 |
| L-Lysine hydrochloride | 183 | 0 | 0 |
| L-Methionine | 149 | 0 | 0 |
| L-Phenylalanine | 165 | 0 | 0 |
| L-Proline | 115 | 0 | 0 |
| L-Serine | 105 | 0 | 0 |
| L-Threonine | 119 | 0 | 0 |
| L-Tryptophan | 204 | 0 | 0 |
| L-Tyrosine | 181 | 0 | 0 |
| L-Valine | 117 | 0 | 0 |
| Vitamins | |||
| Choline chloride | 140 | 0 | 0 |
| D-Pantothenic Acid | 238 | 0 | 0 |
| Folic Acid | 441 | 0 | 0 |
| Myo-Inositol | 180 | 0 | 0 |
| Niacinamide | 122 | 0 | 0 |
| Pyridoxal hydrochloride | 206 | 0 | 0 |
| Riboflavin | 376 | 0 | 0 |
| Thiamine hydrochloride | 337 | 0 | 0 |
| Vitamin B12 | 1355 | 0 | 0 |
| d-Biotin | 244 | 0 | 0 |
| Inorganic Salts | |||
| Ammonium Metavanadate NaVO3 | 117 | 0 | 0 |
| Ammonium Molybdate ((NH4)6Mo7O24-4H2O) | 1236 | 0 | 0 |
| Cupric sulfate (CuSO4-5H2O) | 250 | 0 | 0 |
| Ferric sulfate (FeSO4-7H2O) | 278 | 0 | 0 |
| Magnesium Chloride (MgCl2-6H2O) | 203 | 0 | 0 |
| Manganese Sulfate (MnSO4-H20) | 169 | 0 | 0 |
| Nickelous Chloride NiCl2 6H2O | 238 | 0 | 0 |
| Potassium Chloride (KCl) | 75 | 0 | 0 |
| Sodium Acetate (NaCH3COO.3H2O) | 136 | 0 | 0 |
| Sodium Bicarbonate (NaHCO3) | 84 | 0 | 0 |
| Sodium Chloride (NaCl) | 58 | 0 | 0 |
| Sodium Meta Silicate Na2SiO3 9H2O | 284 | 0 | 0 |
| Sodium Phosphate dibasic (Na2HPO4-7H2O) | 268 | 0 | 0 |
| Sodium Selenite (Na2SeO3) | 173 | 0 | 0 |
| Tin Chloride (SnCl2.2H2O) | 226 | 0 | 0 |
| Zinc sulfate (ZnSO4-7H2O) | 288 | 0 | 0 |
| Other Components | |||
| Adenine.HCl | 172 | 0 | 0 |
| D-Glucose (Dextrose) | 180 | 0 | 0 |
| DL-alpha-Lipoic Acid | 206 | 0 | 0 |
| Ethanolamine | 61 | 0 | 0 |
| HEPES | 238 | 0 | 0 |
| O-Phosphorylethanolamine | 141 | 0 | 0 |
| Phenol Red | 376.4 | 0 | 0 |
| Putrescine 2HCl | 161 | 0 | 0 |
| Sodium Pyruvate | 110 | 0 | 0 |
| Thymidine | 242 | 0 | 0 |
