Technical Resources - Media Formulations
EpiLife® Medium
Without calcium or phenol red.
A sterile, liquid medium prepared without calcium chloride and phenol red for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of calcium plus an appropriate supplement prior to use. Calcium chloride is provided as a separate component with each bottle of medium. Below is the composition list. The concentrations are proprietary.
Catalog Number(s):
MEPICFPRF500
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 0 | 0 |
| L-Alanine | 89 | 0 | 0 |
| L-Arginine hydrochloride | 211 | 0 | 0 |
| L-Asparagine-H2O | 150 | 0 | 0 |
| L-Aspartic acid | 133 | 0 | 0 |
| L-Cysteine | 121 | 0 | 0 |
| L-Glutamic Acid | 147 | 0 | 0 |
| L-Glutamine | 146 | 0 | 0 |
| L-Histidine hydrochloride-H2O | 210 | 0 | 0 |
| L-Isoleucine | 131 | 0 | 0 |
| L-Leucine | 131 | 0 | 0 |
| L-Lysine hydrochloride | 183 | 0 | 0 |
| L-Methionine | 149 | 0 | 0 |
| L-Phenylalanine | 165 | 0 | 0 |
| L-Proline | 115 | 0 | 0 |
| L-Serine | 105 | 0 | 0 |
| L-Threonine | 119 | 0 | 0 |
| L-Tryptophan | 204 | 0 | 0 |
| L-Tyrosine | 181 | 0 | 0 |
| L-Valine | 117 | 0 | 0 |
| Vitamins | |||
| Choline chloride | 140 | 0 | 0 |
| D-Pantothenic Acid | 238 | 0 | 0 |
| Folic Acid | 441 | 0 | 0 |
| Myo-Inositol | 180 | 0 | 0 |
| Niacinamide | 122 | 0 | 0 |
| Pyridoxal hydrochloride | 206 | 0 | 0 |
| Riboflavin | 376 | 0 | 0 |
| Thiamine hydrochloride | 337 | 0 | 0 |
| Vitamin B12 | 1355 | 0 | 0 |
| d-Biotin | 244 | 0 | 0 |
| Inorganic Salts | |||
| Ammonium Metavanadate NaVO3 | 117 | 0 | 0 |
| Ammonium Molybdate ((NH4)6Mo7O24-4H2O) | 1236 | 0 | 0 |
| Cupric sulfate (CuSO4-5H2O) | 250 | 0 | 0 |
| Ferric sulfate (FeSO4-7H2O) | 278 | 0 | 0 |
| Magnesium Chloride (MgCl2-6H2O) | 203 | 0 | 0 |
| Manganese Sulfate (MnSO4-H20) | 169 | 0 | 0 |
| Nickelous Chloride NiCl2 6H2O | 238 | 0 | 0 |
| Potassium Chloride (KCl) | 75 | 0 | 0 |
| Sodium Bicarbonate (NaHCO3) | 84 | 0 | 0 |
| Sodium Chloride (NaCl) | 58 | 0 | 0 |
| Sodium Meta Silicate Na2SiO3 9H2O | 284 | 0 | 0 |
| Sodium Phosphate dibasic (Na2HPO4-7H2O) | 268 | 0 | 0 |
| Sodium Selenite (Na2SeO3) | 173 | 0 | 0 |
| Tin Chloride (SnCl2.2H2O) | 226 | 0 | 0 |
| Zinc sulfate (ZnSO4-7H2O) | 288 | 0 | 0 |
| Other Components | |||
| Adenine.HCl | 172 | 0 | 0 |
| D-Glucose (Dextrose) | 180 | 0 | 0 |
| DL-alpha-Lipoic Acid | 206 | 0 | 0 |
| Ethanolamine | 61 | 0 | 0 |
| HEPES | 238 | 0 | 0 |
| O-Phosphorylethanolamine | 141 | 0 | 0 |
| Putrescine 2HCl | 161 | 0 | 0 |
| Sodium Pyruvate | 110 | 0 | 0 |
| Thymidine | 242 | 0 | 0 |
