Technical Resources - Media Formulations

12557 - PB-MAX™ Karyotyping Medium

Catalog Number(s):
12557013 ,12557021

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 10 0.133
L-Arginine 174 200 1.15
L-Asparagine 132 50 0.379
L-Aspartic acid 133 20 0.15
L-Cystine 2HCl 313 65 0.208
L-Glutamic Acid 147 20 0.136
L-Glutamine 146 300 2.05
L-Histidine 155 15 0.0968
L-Hydroxyproline 131 20 0.153
L-Isoleucine 131 50 0.382
L-Leucine 131 50 0.382
L-Lysine hydrochloride 183 40 0.219
L-Methionine 149 15 0.101
L-Phenylalanine 165 15 0.0909
L-Proline 115 20 0.174
L-Serine 105 30 0.286
L-Threonine 119 20 0.168
L-Tryptophan 204 5 0.0245
L-Tyrosine disodium salt dihydrate 261 29 0.111
L-Valine 117 20 0.171
Vitamins
Biotin 244 0.2 0.00082
Choline chloride 140 3 0.0214
D-Calcium pantothenate 477 0.25 0.000524
Folic Acid 441 1 0.00227
Niacinamide 122 1 0.0082
Para-Aminobenzoic Acid 137 1 0.0073
Pyridoxine hydrochloride 206 1 0.00485
Riboflavin 376 0.2 0.000532
Thiamine hydrochloride 337 1 0.00297
Vitamin B12 1355 0.005 0.0000037
i-Inositol 180 35 0.194
Inorganic Salts
Calcium nitrate (Ca(NO3)2 4H2O) 236 100 0.424
Magnesium Sulfate (MgSO4) (anhyd.) 120 48.84 0.407
Potassium Chloride (KCl) 75 400 5.33
Sodium Bicarbonate (NaHCO3) 84 2000 23.81
Sodium Chloride (NaCl) 58 6000 103.45
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 800 5.63
Other Components
D-Glucose (Dextrose) 180 2000 11.11
FBS 0 �
Gentimicin Sulfate 35 ∞
Glutathione (reduced) 307 1 0.00326
Phenol Red 376.4 5 0.0133
Phytohemagglutinin M 0 �

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