Technical Resources - Media Formulations

31415 - Leibovitz's L-15

Catalog Number(s):
31415029 ,31415086

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 200 2.67
L-Alanine 89 225 2.53
L-Alanyl-L-Glutamine 217 446 2.06
L-Arginine 174 500 2.87
L-Asparagine 132 250 1.89
L-Cysteine 121 120 0.992
L-Histidine 155 250 1.61
L-Isoleucine 131 250 1.91
L-Leucine 131 125 0.954
L-Lysine 146 75 0.514
L-Methionine 149 75 0.503
L-Phenylalanine 165 125 0.758
L-Serine 105 200 1.9
L-Threonine 119 300 2.52
L-Tryptophan 204 20 0.098
L-Tyrosine 181 300 1.66
L-Valine 117 100 0.855
Vitamins
Choline chloride 140 1 0.00714
D-Calcium pantothenate 477 1 0.0021
Folic Acid 441 1 0.00227
Niacinamide 122 1 0.0082
Pyridoxine hydrochloride 206 1 0.00485
Riboflavin 5'-phosphate Na 478 0.1 0.000209
Thiamine monophosphate 442 1 0.00226
i-Inositol 180 2 0.0111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 140 1.26
Magnesium Chloride (anhydrous) 95 93.7 0.986
Magnesium Sulfate (MgSO4) (anhyd.) 120 97.67 0.814
Potassium Chloride (KCl) 75 400 5.33
Potassium Phosphate monobasic (KH2PO4) 136 60 0.441
Sodium Chloride (NaCl) 58 8000 137.93
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 190 1.34
Other Components
D+ Galactose 180 900 5
Phenol Red 376.4 10 0.0266
Sodium Pyruvate 110 550 5

Reference:

  1. Beall, P.T., Karnaky, K.J., Garretson, L.T. et al. (1983) Biochem. Biophys. Acta 763:19 (rat jejumum).
  2. Chinchar, G.D. and Sinclair, J.H. (1978) J. Cell. Physiol. 96:333 (amphibian, frog, ICR, 2A).
  3. Flamme, I., Albach, K., Muller, S. et al. (1991) Anat. Record, 229:427 (chick embryos).
  4. Foxworthy, P.S. and Eacho, P.I. (1986) Toxicol. Ltrs., 30:189 (Rat hepatocytes).
  5. Grothe, C., Otto, D. and Unsicker, K. (1989) Neuroscience 31:649 (Rat embryonic E18 septal neurons).
  6. Leibovitz, A. (1963) Am. J. Hygiene 78:173.
  7. Matsuda, M. (1983) Develop. Biol., 95:219. (Amphibian [newt] neural plates).
  8. Middlebrooks, B.L., Mak, P.C. and Ellender, R.D. (1980) Proc. Soc. Exper. Biol. Med., 165:123 (fish fibroblast).
  9. Powell, R.L., Dodson, M.V. and Cloud, J.G. (1989) J. Exper. Zool., 50:333 (Fish Satellite cells, mygenic stem cells).
  10. Saad, a.H. and El Ridi, R. (1988) Vet. Immunol Imunopath., 18:369 (reptile [lizard] spleen cells).
  11. Tate, J.M., Oberpriller, J.O. and Oberpriller, J.C. (1989) Tissue and Cell, 21:335 (Newt cardiac monocytes).
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