Technical Resources - Media Formulations

22100 - Glasgow's MEM, powder

Catalog Number(s):
22100093

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211 42 0.199
L-Cystine 2HCl 313 31 0.099
L-Glutamine 146 292 2
L-Histidine hydrochloride-H2O 210 21 0.1
L-Isoleucine 131 52 0.397
L-Leucine 131 52 0.397
L-Lysine hydrochloride 183 73 0.399
L-Methionine 149 15 0.101
L-Phenylalanine 165 33 0.2
L-Threonine 119 47.6 0.4
L-Tryptophan 204 8 0.0392
L-Tyrosine disodium salt dihydrate 261 52 0.199
L-Valine 117 46.8 0.4
Vitamins
Choline chloride 140 2 0.0143
D-Calcium pantothenate 477 2 0.00419
Folic Acid 441 2 0.00454
Niacinamide 122 2 0.0164
Pyridoxal hydrochloride 204 2 0.0098
Riboflavin 376 0.2 0.000532
Thiamine hydrochloride 337 2 0.00593
i-Inositol 180 3.6 0.02
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 200 1.8
Ferric Nitrate (Fe(NO3)3"9H2O) 404 0.1 0.000248
Magnesium Sulfate (MgSO4-7H2O) 120 97.67 0.814
Potassium Chloride (KCl) 75 400 5.33
Sodium Chloride (NaCl) 58 6400 110.34
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 124 0.899
Other Components
D-Glucose (Dextrose) 180 4500 25
Phenol Red 376.4 15 0.0399

Reference:

  1. House, W., (1964) Medical Research Council, Institute of Virology,  University of Glasgow, Scotland, (November). 
  2. MacPherson, I. and Stoker, M., (1962)  Virology 16:147.
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