Technical Resources - Media Formulations
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 10 | 0.133 |
| L-Alanine | 89 | 8.9 | 0.1 |
| L-Arginine | 174 | 200 | 1.15 |
| L-Asparagine | 132 | 50 | 0.379 |
| L-Aspartic acid | 133 | 20 | 0.15 |
| L-Cystine 2HCl | 313 | 65 | 0.208 |
| L-Glutamic Acid | 147 | 20 | 0.136 |
| L-Histidine | 155 | 15 | 0.0968 |
| L-Hydroxyproline | 131 | 20 | 0.153 |
| L-Isoleucine | 131 | 50 | 0.382 |
| L-Leucine | 131 | 50 | 0.382 |
| L-Lysine hydrochloride | 146 | 40 | 0.274 |
| L-Methionine | 149 | 15 | 0.101 |
| L-Phenylalanine | 165 | 15 | 0.0909 |
| L-Proline | 115 | 20 | 0.174 |
| L-Serine | 105 | 30 | 0.286 |
| L-Threonine | 119 | 20 | 0.168 |
| L-Tryptophan | 204 | 5 | 0.0245 |
| L-Tyrosine disodium salt | 225 | 29 | 0.129 |
| L-Valine | 117 | 20 | 0.171 |
| Vitamins | |||
| Ascorbic Acid phosphate | 289.54 | 2.5 | 0.00863 |
| Biotin | 244 | 0.2 | 0.00082 |
| Choline chloride | 140 | 3 | 0.0214 |
| D-Calcium pantothenate | 477 | 0.25 | 0.000524 |
| Folic Acid | 441 | 1 | 0.00227 |
| Niacinamide | 122 | 1 | 0.0082 |
| Para-Aminobenzoic Acid | 137 | 1 | 0.0073 |
| Pyridoxine hydrochloride | 206 | 1 | 0.00485 |
| Riboflavin | 376 | 0.2 | 0.000532 |
| Thiamine hydrochloride | 337 | 1 | 0.00297 |
| Vitamin B12 | 1355 | 0.005 | 0.0000037 |
| i-Inositol | 180 | 35 | 0.194 |
| Inorganic Salts | |||
| Calcium nitrate (Ca(NO3)2 4H2O) | 236 | 100 | 0.424 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 48.84 | 0.407 |
| Potassium Chloride (KCl) | 75 | 400 | 5.33 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2000 | 23.81 |
| Sodium Chloride (NaCl) | 58 | 6000 | 103.45 |
| Sodium Phosphate dibasic (Na2HPO4) anhydrous | 142 | 800 | 5.63 |
| Zinc sulfate (ZnSO4-7H2O) | 288 | 0.874 | 0.00303 |
| Proteins | |||
| AlbuMAX® II | 400 | ∞ | |
| Human Transferrin (Holo) | 7.5 | ∞ | |
| Insulin Recombinant Full Chain | 10 | ∞ | |
| Trace Elements | |||
| Ammonium Metavanadate | 116.98 | 0.0003 | 0.0000026 |
| Cupric Sulfate | 250 | 0.00125 | 0.000005 |
| Manganous Chloride | 198 | 0.00005 | 0.0000003 |
| Sodium Selenite | 173 | 0.005 | 0.0000289 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 2000 | 11.11 |
| Ethanolamine | 97.54 | 1.9 | 0.0195 |
| Glutathione (reduced) | 307 | 1 | 0.00326 |
| Phenol Red | 376.4 | 5 | 0.0133 |
| Sodium Pyruvate | 110 | 110 | 1 |
