Technical Resources - Media Formulations
CMRL Medium-1066 (10X) liquid
Contains no L-glutamine or sodium bicarbonate
CMRL media are well suited for the growth of Earle's "L" cells. They are also especially useful for cloning monkey kidney cell cultures and for growth of other mammalian cell lines when enriched with horse or calf serum. CMRL 1066 is particularly rich in nucleosides and some vitamins.
Catalog Number(s):
21540026
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 500 | 6.67 |
| Hydroxy L-proline | 131 | 100 | 0.763 |
| L-Alanine | 89 | 250 | 2.81 |
| L-Arginine hydrochloride | 211 | 700 | 3.32 |
| L-Aspartic acid | 133 | 300 | 2.26 |
| L-Cysteine | 121 | 1998.8 | 16.52 |
| L-Cystine | 240 | 200 | 0.833 |
| L-Glutamic Acid | 147 | 750 | 5.1 |
| L-Histidine hydrochloride-H2O | 210 | 200 | 0.952 |
| L-Isoleucine | 131 | 200 | 1.53 |
| L-Leucine | 131 | 600 | 4.58 |
| L-Lysine hydrochloride | 183 | 700 | 3.83 |
| L-Methionine | 149 | 150 | 1.01 |
| L-Phenylalanine | 165 | 250 | 1.52 |
| L-Proline | 115 | 400 | 3.48 |
| L-Serine | 105 | 250 | 2.38 |
| L-Threonine | 119 | 300 | 2.52 |
| L-Tryptophan | 204 | 100 | 0.49 |
| L-Tyrosine | 181 | 400 | 2.21 |
| L-Valine | 117 | 250 | 2.14 |
| Vitamins | |||
| Ascorbic Acid | 176 | 500 | 2.84 |
| Biotin | 244 | 0.1 | 0.00041 |
| Cholesterol | 387 | 2 | 0.00517 |
| Choline chloride | 140 | 5 | 0.0357 |
| D-Calcium pantothenate | 477 | 0.1 | 0.00021 |
| Folic Acid | 441 | 0.1 | 0.000227 |
| Niacinamide | 122 | 0.25 | 0.00205 |
| Nicotinic acid (Niacin) | 123 | 0.25 | 0.00203 |
| Para-Aminobenzoic Acid | 137 | 0.5 | 0.00365 |
| Pyridoxine hydrochloride | 206 | 0.25 | 0.00121 |
| Riboflavin | 376 | 0.1 | 0.000266 |
| Thiamine hydrochloride | 337 | 0.1 | 0.000297 |
| i-Inositol | 180 | 0.5 | 0.00278 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2-2H2O) | 147 | 2000 | 13.61 |
| Magnesium Sulfate (MgSO4-7H2O) | 246 | 2000 | 8.13 |
| Potassium Chloride (KCl) | 75 | 4000 | 53.33 |
| Sodium Chloride (NaCl) | 58 | 6799 | 117.22 |
| Sodium Phosphate monobasic (NaH2PO4-2H2O) | 156 | 1580 | 10.13 |
| Other Components | |||
| 2'Deoxyadenosine | 251 | 100 | 0.398 |
| 2'Deoxycytidine | 227 | 100 | 0.441 |
| 2'Deoxyguanosine | 267 | 100 | 0.375 |
| 5-Methyl-deoxycytidine | 225 | 1 | 0.00444 |
| Co-carboxylase | 461 | 10 | 0.0217 |
| Coenzyme A | 768 | 25 | 0.0326 |
| D-Glucose (Dextrose) | 180 | 10000 | 55.56 |
| Diphosphopyridine nucleotide (NAD) | 663 | 70 | 0.106 |
| FAD (flavin adenine dinucleotide) | 786 | 10 | 0.0127 |
| Glutathione (reduced) | 307 | 100 | 0.326 |
| Phenol Red | 376.4 | 200 | 0.531 |
| Sodium acetate-3H2O | 136 | 830 | 6.1 |
| Sodium glucuronate-H2O | 236 | 40 | 0.169 |
| Thymidine | 242 | 100 | 0.413 |
| Triphosphopyridine Nucleotide (NADP) | 743 | 10 | 0.0135 |
| Tween 80® | 50 | ∞ | |
| Uridine 5'- triphosphate | 484 | 10 | 0.0207 |
Reference:
- Parker R.C., et al. (1957) Special Publications, N.Y. Academy of Sciences, 5, 303.
