Technical Resources - Media Formulations

31870 - RPMI 1640, no glutamine

Catalog Number(s):
31870025 ,31870074

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 10 0.133
L-Arginine 174 200 1.15
L-Asparagine 132 50 0.379
L-Aspartic acid 133 20 0.15
L-Cystine 240 50 0.208
L-Glutamic Acid 147 20 0.136
L-Histidine 155 15 0.0968
L-Hydroxyproline 131 20 0.153
L-Isoleucine 131 50 0.382
L-Leucine 131 50 0.382
L-Lysine hydrochloride 146 40 0.274
L-Methionine 149 15 0.101
L-Phenylalanine 165 15 0.0909
L-Proline 115 20 0.174
L-Serine 105 30 0.286
L-Threonine 119 20 0.168
L-Tryptophan 204 5 0.0245
L-Tyrosine 181 20 0.11
L-Valine 117 20 0.171
Vitamins
Biotin 244 0.2 0.00082
Choline chloride 140 3 0.0214
D-Calcium pantothenate 477 0.25 0.000524
Folic Acid 441 1 0.00227
Niacinamide 122 1 0.0082
Para-Aminobenzoic Acid 137 1 0.0073
Pyridoxine hydrochloride 206 1 0.00485
Riboflavin 376 0.2 0.000532
Thiamine hydrochloride 337 1 0.00297
Vitamin B12 1355 0.005 0.0000037
i-Inositol 180 35 0.194
Inorganic Salts
Calcium nitrate (Ca(NO3)2 4H2O) 236 100 0.424
Magnesium Sulfate (MgSO4-7H2O) 246 100 0.407
Potassium Chloride (KCl) 75 400 5.33
Sodium Bicarbonate (NaHCO3) 84 2000 23.81
Sodium Chloride (NaCl) 58 6000 103.45
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 800 5.63
Other Components
D-Glucose (Dextrose) 180 2000 11.11
Glutathione (reduced) 307 1 0.00326
Phenol Red 376.4 5 0.0133

Reference:

  1. Moore, G.E., Gerner, R.E. and Franklin, H.A. (1967) J.A.M.A., 199:519.
  2. Mossinger, J. (1991) Parasitol., 103:85 (Nematodes, Litomosoides carinii).
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