Technical Resources - Media Formulations

G-5 Supplement (100X) liquid

For the growth and expression of glial cells (normal and tumor) of astrocytic phenotype.

G-5 Supplement is a chemically defined, 100X concentrate of Bottenstein G-5 formulation (1). This Supplement is designed for the growth and expression of primary glial cells(astrocytes) or tumor cell lines of astrocytic phenotype(astrocytes and gliomas). It can be used for continuous cell lines. It can be used as a substitute for the G-2 Bottenstein formulation.

Catalog Number(s):
17503012

Components Molecular Weight Concentration (mg/L) mM
Vitamins
Biotin 244 100 0.41
Proteins
Basic FGF 0.5 ∞
EGF 1 ∞
Human Transferrin 5000 ∞
Insulin 500 ∞
Other Components
Hydrocortisone 362.47 0.36 0.000993
Selenite 173 0.52 0.00301

Reference:

  1. Bottenstein, J.E. (1985) Cell Culture in the Neurosciences, Bottenstein, J.E. and Harvey, A.L., editors, p. 3, Plenum Press: New York and London.
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