Technical Resources - Media Formulations

14175 - HBSS, no calcium, no magnesium, no phenol red

Catalog Number(s):
14175046 ,14175053 ,14175079 ,14175095 ,14175103 ,14175129 ,14175137 ,14175145

Components Molecular Weight Concentration (mg/L) mM
Inorganic Salts
Potassium Chloride (KCl) 75 400 5.33
Potassium Phosphate monobasic (KH2PO4) 136 60 0.441
Sodium Bicarbonate (NaHCO3) 84 350 4.17
Sodium Chloride (NaCl) 58 8000 137.93
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 48 0.338
Other Components
D-Glucose (Dextrose) 180 1000 5.56

Reference:

  1. Hanks, J.H. and Wallace, R.E. (1949) Relation of Oxygen and Temperature in the Preservation of Tissues by Refrigeration. Proc. Soc. Exp. Biol. Med., 71:196.