Technical Resources - Media Formulations
14065 - HBSS, 10x, calcium, magnesium, no phenol red
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 1400 | 12.61 |
| Magnesium Chloride (MgCl2-6H2O) | 203 | 1000 | 4.93 |
| Magnesium Sulfate (MgSO4-7H2O) | 246 | 1000 | 4.07 |
| Potassium Chloride (KCl) | 75 | 4000 | 53.33 |
| Potassium Phosphate monobasic (KH2PO4) | 136 | 600 | 4.41 |
| Sodium Chloride (NaCl) | 58 | 80000 | 1379.31 |
| Sodium Phosphate dibasic (Na2HPO4-7H2O) | 268 | 900 | 3.36 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 10000 | 55.56 |
Reference:
- Hanks, J.H. and Wallace, R.E. (1949) Relation of Oxygen and Temperature in the Preservation of Tissues by Refrigeration. Proc. Soc. Exp. Biol. Med., 71:196.
