Technical Resources - Media Formulations

11220 - Waymouth's

Catalog Number(s):
11220035

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 50 0.667
L-Arginine hydrochloride 211 75 0.355
L-Aspartic acid 133 60 0.451
L-Cysteine 121 61 0.504
L-Cystine 240 15 0.0625
L-Glutamic Acid 147 150 1.02
L-Glutamine 146 350 2.4
L-Histidine 155 128 0.826
L-Isoleucine 131 25 0.191
L-Leucine 131 50 0.382
L-Lysine hydrochloride 183 240 1.31
L-Methionine 149 50 0.336
L-Phenylalanine 165 50 0.303
L-Proline 115 50 0.435
L-Threonine 119 75 0.63
L-Tryptophan 204 40 0.196
L-Tyrosine 181 40 0.221
L-Valine 117 65 0.556
Vitamins
Ascorbic Acid 176 17.5 0.0994
Biotin 244 0.02 0.000082
Choline chloride 140 250 1.79
D-Calcium pantothenate 477 1 0.0021
Folic Acid 441 0.5 0.00113
Niacinamide 122 1 0.0082
Pyridoxine hydrochloride 206 1 0.00485
Riboflavin 376 1 0.00266
Thiamine hydrochloride 337 10 0.0297
Vitamin B12 1355 0.2 0.000148
i-Inositol 180 1 0.00556
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147 120 0.816
Magnesium Chloride (MgCl2-6H2O) 203 240 1.18
Magnesium Sulfate (MgSO4-7H2O) 246 200 0.813
Potassium Chloride (KCl) 75 150 2
Potassium Phosphate monobasic (KH2PO4) 136 80 0.588
Sodium Bicarbonate (NaHCO3) 84 2240 26.67
Sodium Chloride (NaCl) 58 6000 103.45
Sodium Phosphate dibasic (Na2HPO4-7H2O) 268 566 2.11
Other Components
D-Glucose (Dextrose) 180 5000 27.78
Glutathione (reduced) 307 15 0.0489
Hypoxanthine 136 25 0.184
Phenol Red 376.4 10 0.0266

Reference:

  1. Waymouth, C. (1968) personal communication.
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