Technical Resources - Media Formulations
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 10 | 0.133 |
| L-Arginine | 174 | 200 | 1.15 |
| L-Asparagine | 132 | 50 | 0.379 |
| L-Aspartic acid | 133 | 20 | 0.15 |
| L-Cystine 2HCl | 313 | 65 | 0.208 |
| L-Glutamic Acid | 147 | 20 | 0.136 |
| L-Glutamine | 146 | 300 | 2.05 |
| L-Histidine | 155 | 15 | 0.0968 |
| L-Hydroxyproline | 131 | 20 | 0.153 |
| L-Isoleucine | 131 | 50 | 0.382 |
| L-Leucine | 131 | 50 | 0.382 |
| L-Lysine hydrochloride | 183 | 40 | 0.219 |
| L-Methionine | 149 | 15 | 0.101 |
| L-Phenylalanine | 165 | 15 | 0.0909 |
| L-Proline | 115 | 20 | 0.174 |
| L-Serine | 105 | 30 | 0.286 |
| L-Threonine | 119 | 20 | 0.168 |
| L-Tryptophan | 204 | 5 | 0.0245 |
| L-Tyrosine disodium salt dihydrate | 261 | 29 | 0.111 |
| L-Valine | 117 | 20 | 0.171 |
| Vitamins | |||
| Biotin | 244 | 0.2 | 0.00082 |
| Choline chloride | 140 | 3 | 0.0214 |
| D-Calcium pantothenate | 477 | 0.25 | 0.000524 |
| Folic Acid | 441 | 1 | 0.00227 |
| Niacinamide | 122 | 1 | 0.0082 |
| Para-Aminobenzoic Acid | 137 | 1 | 0.0073 |
| Pyridoxine hydrochloride | 206 | 1 | 0.00485 |
| Riboflavin | 376 | 0.2 | 0.000532 |
| Thiamine hydrochloride | 337 | 1 | 0.00297 |
| Vitamin B12 | 1355 | 0.005 | 0.0000037 |
| i-Inositol | 180 | 35 | 0.194 |
| Inorganic Salts | |||
| Calcium nitrate (Ca(NO3)2 4H2O) | 236 | 100 | 0.424 |
| Magnesium Sulfate (MgSO4-7H2O) | 246 | 48.84 | 0.199 |
| Potassium Chloride (KCl) | 75 | 400 | 5.33 |
| Sodium Chloride (NaCl) | 58 | 6000 | 103.45 |
| Sodium Phosphate dibasic (Na2HPO4-7H2O) | 268 | 800 | 2.99 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 2000 | 11.11 |
| Glutathione (reduced) | 307 | 1 | 0.00326 |
| Phenol Red | 376.4 | 5 | 0.0133 |
Reference:
- Moore, G.E., Gerner, R.E. and Franklin, H.A. (1967) A.M.A., 199, 519.
