Technical Resources - Media Formulations
21340 - NCTC-109, no Glutamine
Catalog Number(s):
21340039
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 13.6 | 0.181 |
| Hydroxy L-proline | 131 | 4.09 | 0.0312 |
| L-Alanine | 89 | 31.6 | 0.355 |
| L-Arginine hydrochloride | 211 | 31.16 | 0.148 |
| L-Asparagine-H2O | 150 | 9.17 | 0.0611 |
| L-Aspartic acid | 133 | 9.91 | 0.0745 |
| L-Cysteine hydrochloride-H2O | 176 | 290 | 1.65 |
| L-Cystine 2HCl | 313 | 14 | 0.0447 |
| L-Glutamic Acid | 147 | 8.26 | 0.0562 |
| L-Histidine hydrochloride-H2O | 210 | 26.65 | 0.127 |
| L-Isoleucine | 131 | 18.04 | 0.138 |
| L-Leucine | 131 | 20.44 | 0.156 |
| L-Lysine hydrochloride | 183 | 38.43 | 0.21 |
| L-Methionine | 149 | 4.44 | 0.0298 |
| L-Ornithine HCl | 169 | 9.41 | 0.0557 |
| L-Phenylalanine | 165 | 16.53 | 0.1 |
| L-Proline | 115 | 6.13 | 0.0533 |
| L-Serine | 105 | 10.75 | 0.102 |
| L-Taurine | 125 | 4.18 | 0.0334 |
| L-Threonine | 119 | 18.93 | 0.159 |
| L-Tryptophan | 204 | 17.5 | 0.0858 |
| L-Tyrosine disodium salt dihydrate | 181 | 21 | 0.116 |
| L-Valine | 117 | 25 | 0.214 |
| Vitamins | |||
| Biotin | 244 | 0.025 | 0.000102 |
| Choline chloride | 140 | 1.25 | 0.00893 |
| D-Calcium pantothenate | 477 | 0.025 | 0.0000524 |
| Folic Acid | 441 | 0.025 | 0.0000567 |
| Menadione (Vitamin K3) | 172 | 0.025 | 0.000145 |
| Niacinamide | 122 | 0.0625 | 0.000512 |
| Nicotinic acid (Niacin) | 123 | 0.0625 | 0.000508 |
| Para-Aminobenzoic Acid | 137 | 0.125 | 0.000912 |
| Pyridoxal hydrochloride | 204 | 0.0625 | 0.000306 |
| Pyridoxine hydrochloride | 206 | 0.0625 | 0.000303 |
| Riboflavin | 376 | 0.025 | 0.0000665 |
| Thiamine hydrochloride | 337 | 0.025 | 0.0000742 |
| Vitamin A (acetate) | 286 | 0.25 | 0.000874 |
| Vitamin B12 | 1355 | 10 | 0.00738 |
| Vitamin D2 (Calciferol) | 397 | 0.25 | 0.00063 |
| alpha Tocopherol phos. Na salt | 702 | 0.025 | 0.0000356 |
| i-Inositol | 180 | 0.125 | 0.000694 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 200 | 1.8 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 97.67 | 0.814 |
| Potassium Chloride (KCl) | 75 | 400 | 5.33 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2200 | 26.19 |
| Sodium Chloride (NaCl) | 58 | 6800 | 117.24 |
| Sodium Phosphate monobasic (NaH2PO4-H2O) | 138 | 140 | 1.01 |
| Ribonucleosides | |||
| 5-Methylcytosine | 225 | 0.1 | 0.000444 |
| Deoxyribonucleosides | |||
| 2'Deoxyadenosine | 251 | 10 | 0.0398 |
| 2'Deoxycytidine HCl | 264 | 10 | 0.0379 |
| 2'Deoxyguanosine | 267 | 10 | 0.0375 |
| Thymidine | 242 | 10 | 0.0413 |
| Coenzymes | |||
| CoA (Coenzyme A) | 768 | 2.5 | 0.00326 |
| DPN (diphosphopyridine nucleotide) | 663 | 7 | 0.0106 |
| FAD (flavin adenine dinucleotide) | 786 | 1 | 0.00127 |
| TPN (triphosphopyridine nucleotide-Na) | 743 | 1 | 0.00135 |
| TPP Co-carboxylase | 461 | 1 | 0.00217 |
| UTP (uridine triphosphate 3Na-2H20) | 586 | 1 | 0.00171 |
| Reducing Agents | |||
| Ascorbic Acid | 176 | 50 | 0.284 |
| Glutathione, monosodium | 329 | 10 | 0.0304 |
| Other Components | |||
| D-Glucosamine HCl | 216 | 3.85 | 0.0178 |
| D-Glucose (Dextrose) | 180 | 1000 | 5.56 |
| D-Glucuronolactone | 176 | 1.8 | 0.0102 |
| L-alpha-Amino-n-butyric acid | 103 | 5.5 | 0.0534 |
| Phenol Red | 376.4 | 20 | 0.0531 |
| Sodium Acetate | 136 | 50 | 0.368 |
| Sodium glucuronate-H2O | 236 | 1.8 | 0.00763 |
| Tween 80® | 12.5 | ∞ | |
