Technical Resources - Media Formulations

32571 - MEM alpha, GlutaMAX(TM), nucleosides

Catalog Number(s):
32571028 ,32571036 ,32571093 ,32571101

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 50 0.667
L-Alanine 89 25 0.281
L-Alanyl-L-Glutamine 203 406 2
L-Arginine 211 105 0.498
L-Asparagine-H2O 132 50 0.379
L-Aspartic acid 133 30 0.226
L-Cysteine hydrochloride 121 100 0.826
L-Cystine 313 31 0.099
L-Glutamic Acid 147 75 0.51
L-Histidine 155 31 0.2
L-Isoleucine 131 52.4 0.4
L-Leucine 131 52.4 0.4
L-Lysine 146 58 0.397
L-Methionine 149 15 0.101
L-Phenylalanine 165 32 0.194
L-Proline 115 40 0.348
L-Serine 105 25 0.238
L-Threonine 119 48 0.403
L-Tryptophan 204 10 0.049
L-Tyrosine 181 36 0.199
L-Valine 117 46 0.393
Vitamins
Ascorbic Acid 176 50 0.284
Biotin 244 0.1 0.00041
Choline chloride 140 1 0.00714
D-Calcium pantothenate 477 1 0.0021
Folic Acid 441 1 0.00227
Niacinamide 122 1 0.0082
Pyridoxal hydrochloride 204 1 0.0049
Riboflavin 376 0.1 0.000266
Thiamine hydrochloride 337 1 0.00297
Vitamin B12 1355 1.36 0.001
i-Inositol 180 2 0.0111
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147 264 1.8
Magnesium Sulfate (MgSO4-7H2O) 246 200 0.813
Potassium Chloride (KCl) 75 400 5.33
Sodium Bicarbonate (NaHCO3) 84 2200 26.19
Sodium Chloride (NaCl) 58 6800 117.24
Sodium Phosphate monobasic (NaH2PO4-2H2O) 156 158 1.01
Ribonucleosides
Adenosine 267 10 0.0375
Cytidine 243 10 0.0412
Guanosine 283 10 0.0353
Uridine 244 10 0.041
Deoxyribonucleosides
2'Deoxyadenosine 251 10 0.0398
2'Deoxycytidine HCl 264 10 0.0379
2'Deoxyguanosine 267 10 0.0375
Thymidine 242 10 0.0413
Other Components
D-Glucose (Dextrose) 180 1000 5.56
Lipoic Acid 206 0.2 0.000971
Phenol Red 376.4 10 0.0266
Sodium Pyruvate 110 110 1

Reference:

  1. Simonsen, C.C. and Levinson, A.D. 1983. Isolation and expression of an altered mouse dihydrofolate reductase cDNA. Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499.
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