Technical Resources - Media Formulations

32561 - MEM alpha, GlutaMAX(TM), no nucleosides

Catalog Number(s):
32561029 ,32561037 ,32561094 ,32561102

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 50 0.667
L-Alanine 89 25 0.281
L-Alanyl-L-Glutamine 203 406 2
L-Arginine 211 105 0.498
L-Asparagine-H2O 132 50 0.379
L-Aspartic acid 133 30 0.226
L-Cysteine hydrochloride 158 100 0.633
L-Cystine 240 24 0.1
L-Glutamic Acid 147 75 0.51
L-Histidine 155 31 0.2
L-Isoleucine 131 52.4 0.4
L-Leucine 131 52.4 0.4
L-Lysine 146 58 0.397
L-Methionine 149 15 0.101
L-Phenylalanine 165 32 0.194
L-Proline 115 40 0.348
L-Serine 105 25 0.238
L-Threonine 119 48 0.403
L-Tryptophan 204 10 0.049
L-Tyrosine 181 36 0.199
L-Valine 117 46 0.393
Vitamins
Ascorbic Acid 176 50 0.284
Biotin 244 0.1 0.00041
Choline chloride 140 1 0.00714
D-Calcium pantothenate 477 1 0.0021
Folic Acid 441 1 0.00227
Niacinamide 122 1 0.0082
Pyridoxal hydrochloride 204 1 0.0049
Riboflavin 376 0.1 0.000266
Thiamine hydrochloride 337 1 0.00297
Vitamin B12 1355 1.36 0.001
i-Inositol 180 2 0.0111
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147 264 1.8
Magnesium Sulfate (MgSO4-7H2O) 246 200 0.813
Potassium Chloride (KCl) 75 400 5.33
Sodium Bicarbonate (NaHCO3) 84 2200 26.19
Sodium Chloride (NaCl) 58 6800 117.24
Sodium Phosphate monobasic (NaH2PO4-2H2O) 156 158 1.01
Other Components
D-Glucose (Dextrose) 180 1000 5.56
Lipoic Acid 206 0.2 0.000971
Phenol Red 376.4 10 0.0266
Sodium Pyruvate 110 110 1

Reference:

  1. Simonsen, C.C. and Levinson, A.D. 1983. Isolation and expression of an altered mouse dihydrofolate reductase cDNA. Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499.
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