Technical Resources - Media Formulations
12360 - MEM, HEPES, no glutamine
Catalog Number(s):
12360038
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| L-Arginine hydrochloride | 211 | 126 | 0.597 |
| L-Cystine 2HCl | 313 | 31 | 0.099 |
| L-Histidine hydrochloride-H2O | 210 | 42 | 0.2 |
| L-Isoleucine | 131 | 52 | 0.397 |
| L-Leucine | 131 | 52 | 0.397 |
| L-Lysine hydrochloride | 183 | 73 | 0.399 |
| L-Methionine | 149 | 15 | 0.101 |
| L-Phenylalanine | 165 | 32 | 0.194 |
| L-Threonine | 119 | 48 | 0.403 |
| L-Tryptophan | 204 | 10 | 0.049 |
| L-Tyrosine disodium salt dihydrate | 261 | 52 | 0.199 |
| L-Valine | 117 | 46 | 0.393 |
| Vitamins | |||
| Choline chloride | 140 | 1 | 0.00714 |
| D-Calcium pantothenate | 477 | 1 | 0.0021 |
| Folic Acid | 441 | 1 | 0.00227 |
| Niacinamide | 122 | 1 | 0.0082 |
| Pyridoxal hydrochloride | 204 | 1 | 0.0049 |
| Riboflavin | 376 | 0.1 | 0.000266 |
| Thiamine hydrochloride | 337 | 1 | 0.00297 |
| i-Inositol | 180 | 2 | 0.0111 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 200 | 1.8 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 97.67 | 0.814 |
| Potassium Chloride (KCl) | 75 | 400 | 5.33 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2200 | 26.19 |
| Sodium Chloride (NaCl) | 58 | 6350 | 109.48 |
| Sodium Phosphate monobasic (NaH2PO4-H2O) | 138 | 140 | 1.01 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 1000 | 5.56 |
| HEPES | 238 | 5958 | 25.03 |
| Phenol Red | 376.4 | 10 | 0.0266 |
Reference:
- Eagle, H. (1959) Science 130:432.
