Technical Resources - Cell Line Database

Cell Lines Detail

Cell Line Name:

Adventitial fibroblasts

Organism Type:

Human

Tissues:

Aorta

Adherence Phenotype:

Adherent

Primary:

Yes

Scientific Application(s):

Cell Culture / Growth Conditions

Comments:

A small part of the horacic aorta was dissected from human donor and recipient hearts during heart transplantation. The adventitial layer was dissected from the aorta and cells were isolated with the use of collagenase. The cells that attached were cultured in DMEM, supplemented with L-glutamine, 100 U/mL penicillin, 100 ug/mL streptomycin, and 10% fetal bovine serum.

 

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