Technical Resources - Cell Line Database

Cell Lines Detail

Cell Line Name:

C127

Organism Type:

Mouse

Tissues:

Epithelial

Adherence Phenotype:

Adherent

Primary:

No

Scientific Application(s):

Stable Cell Transfection

Comments:

This is a cell line derived from mouse mammary epithelial tissues. These cells were infected with a recombinant viruses (10-20 multiplicity of infection each), containing the bacteriophage T7 RNA polymerase driven by a viral promoter. A second recombinant virus containing CFTR cDNA driven by a T7 promoter was added simultaneously, or a plasmid containing the T7 promoter was transfected using Lipofectin 1 h after infection.

 

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