TOPO®

TOPO® Cloning Technology

Ten years ago, Invitrogen launched TOPO® cloning technology and literally sparked a cloning revolution. Over the past decade, TOPO® technology has become the most widely used cloning method in the world. TOPO® cloning technology allows you to perform bench-top cloning reactions in just five minutes—with up to 95% recovery of your desired clone. So you always have the clone you need for downstream experiments.

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Meet the Inventor


Find out how the revolutionary TOPO® cloning was developed with Dr. John Chestnut, research fellow in Synthetic Biology RND.

Dr. Jon Chesnut
TOPO® Cloning Technology