Qubit® Fluorometric Quantitation Technical Resources
 | Find technical resources for your Qubit® 2.0 Fluorometer including a variety of application and technical notes such as Qubit® Quantitation Platform vs. the Nanodrop® Spectrophotometer and Testing for Bias in the Quant-iT™ RNA Assay. Also available are Qubit® frequently asked questions, citations, and valuable customer ratings & reviews. |
Technical Notes
Qubit® Quantitation Platform vs. Spectrophotometer
Testing for Bias in the Qubit® RNA Assay
Single-stranded DNA in the Qubit® dsDNA Assays for the Qubit® Fluorometer
The Use of Glycogen and GlycoBlue™ Reagent in the Qubit® DNA and RNA Assays as Measured on the Qubit® 2.0 Fluorometer
 | Detection and quantitation of nucleic acids are vital to many biological studies. Historically, DNA and RNA have been quantified using spectrophotometry to measure absorbance at 260 nm. Although this method is most commonly used, it can be unreliable and inaccurate.
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Testing for Bias in the Qubit® RNA Assay
 | The Qubit® RNA assay measures RNA concentration in samples containing 250 pg/μL to 100 ng/μL of RNA. To test for the presence and extent of any bias in the Qubit® RNA assay for any of the bases (A, C, G, or U), or for single- or double-stranded RNA, the response of single- and double-stranded RNA homopolymers in the Qubit® RNA assay has been examined. |
Single-stranded DNA in the Qubit® dsDNA Assays for the Qubit® Fluorometer
 | Invitrogen offers two assays for quantifying double-stranded DNA (dsDNA) using the Qubit® Fluorometer: the Qubit® dsDNA HS (for High Sensitivity) Assay and the Qubit® dsDNA BR (for Broad Range) Assay. One advantage of these assays is their specificity for dsDNA. This study was carried out to investigate whether the Qubit® dsDNA quantitation assays for the Qubit® Fluorometer accurately determine the concentration of dsDNA when the samples contain short or long single-stranded DNA (ssDNA). |
The Use of Glycogen and GlycoBlue™ Reagent in the Qubit® DNA and RNA Assays as Measured on the Qubit® 2.0 Fluorometer
 | Glycogen (a branched-chain carbohydrate) and GlycoBlue™ reagent (glycogen covalently linked to a blue dye) are reagents commonly used to facilitate nucleic acid precipitation without adding extraneous nucleic acids to the sample. This study was carried out to determine whether glycogen or GlycoBlue™ reagent affects the accuracy of nucleic acid quantitation using Qubit® kits for the Qubit® 2.0 Fluorometer. |
Qubit® Fluorometer Quick Resources
| Literature Citations See how your colleagues are using the Qubit® 2.0 Fluorometer with our library of references and citations. | FAQs Get the answers to some Frequently Asked Questions about the Qubit® 2.0 Fluorometer.
| Ratings & Reviews See what your peers are saying about the Qubit® Fluorometer by reading customer feedback. |
Qubit® 2.0 Fluorometer: A Researcher's Story
Qubit® Fluorometric Quantitation
Combining the small and economical Qubit® 2.0 Fluorometer with highly sensitive fluorescence-based Qubit® assays.
- Learn More about Qubit® Fluorometric Quantitation
Qubit® 2.0 Fluorometer Resources
Original Qubit® (1.0) Fluorometer Resources
