Qubit® Assays for Use With the Qubit® 2.0 Fluorometer

Easy, Accurate and Sensitive

Qubit® Assay Kits provide concentrated assay reagent, dilution buffer, and prediluted standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. The assay is performed at room temperature, and the signal is stable for 3 hours.

PLEASE NOTE: Qubit® Assay Kits are compatible with both the Qubit® 2.0 Fluorometer and the original Qubit® (1.0) Fluorometer.

Qubit® Assay Kits Selection Guide

Qubit® Assay Kit	Assay Range	Sample Starting Concentration	100 Samples*	500 Samples*
dsDNA HS Assay	0.2–100 ng	10 pg/µL–100 ng/µL	Q32851	Q32854
dsDNA BR Assay	2–1000 ng	100 pg/µL–1 ug/µL	Q32850	Q32853
ssDNA Assay	1–200 ng	50 pg/µL– 200 ng/µL	Q10212	NA
RNA Assay	5–100 ng	250 pg/µL – 100 ng/µL	Q32852	Q32855
RNA BR Assay	20–1000 ng	1 ng/µL–1 µg/µL	Q10210	Q10211
Protein Assay	0.25–5 µg	12.5 µg/mL–5 mg/mL	Q33211	Q33212

* Based on an assay volume of 200 μL. NA = Not Available.

Qubit® Assay Kits Data

(Click for larger view)		Figure 1. Performance of the Qubit® dsDNA HS Assay. The Qubit® dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.
(Click for larger view)		Figure 2. Performance of the Qubit® RNA Assay. The Qubit® RNA Assay has a linear detection range of 5–100 ng and is selective for RNA, even in the presence of an equal mass of DNA.
(Click for larger view)		Figure 3. The Qubit® Protein Assay has a linear detection range of 0.25-5 µg and is selective for protein, even in the presence of an equal mass of DNA or RNA.

Basic Qubit® Fluorometric Quantitation Protocol

1. Prepare dye Working Solution in a plastic tube.
                a.   Use 200 µL of buffer for every sample.
                b.   Use 1 µL of dye for every sample.
                c.   Mix by vortexing.

2. Aliquot 190 µL of Working Solution into two assay tubes for standards (three for the protein assay).

3. Add 10 µL of each Standard to an assay tube and mix by vortexing.

4. Aliquot 180–199 µL of Working Solution into assay tubes for samples.
               a. The assays tolerate 1–20 µL of sample per tube.
               b. The final volume in each tube after adding sample should be 200 µL.

5. Add 1–20 µL of each sample to an assay tube and mix by vortexing.

6. Incubate 2 minutes (15 minutes for the protein assay).

7. Read the results in the Qubit® 2.0 Fluorometer.