- IP Myth 1: Background Can't Be Avoided
- IP Myth 2: PreClearing Is Necessary
- IP Myth 3: Capacity Is Crucial
- IP Myth 4: Dynabeads® Is Expensive
| FACT: Almost all background is removed with Dynabeads® | |||
| Simplicity is the key. Remove all unwanted buffer, but don't disturb/lose your precious sample (animation at right). Fast kinetics with Dynabeads® means fast protocols. And you'll avoid build-up of non-specific binding over time. And obviously, the recombinant protein A/G on the beads have no albumin binding sites. No wonder researchers are moving to magnetic separation for their IP ! Compare IP methods (click to enlarge) | Solution to two typical IP problems: | ||
| FACT: Preclearing is really not necessary with Dynabeads® | ||
Still do preclearing thinking this will help reduce background? | Skip redundant steps! ![]() Four persons each performed two parallells using sepharose (incl. preclearing, SP1-SP4) and magnetic separation (without preclearing, DP1-DP4) . As seen, the yield and reproducibility is significantly better with Dynabeads®. | |
| FACT: High capacity is not always a good thing | ||
You want to balance capacity, yield, specificity, and cost. With Dynabeads®, you get: | Optimize the antigen binding capacity: ![]() The antigen (Ag) capacity is determined by the amount of available antibody (Ab). With Dynabeads®, all the Abs are bound on the surface. There's no excess interior surface, and hence no dead volume to trap Ab, Ag, or impurities. | |
| FACT: You can save money using Dynabeads® | ||
The right capacity and use of your Ab! It's about time the old IP-myths are busted. Scientists are keen to try Dynabeads®. And they love what they see! | Cost Considerations: ![]() As it is difficult to work with volumes lower than 50µl of 50% slurry, the excess capacity cannot be fully utilized (see Myth 3). Slurry is cheap. As shown, the price per sample when using Dynabeads® is comparable with 1-10 µg of Ab, and HALF THE PRICE if pre-clearing with Sepharose®/agarose is done. | |
Choose Your Product:Interactive Selection Guide to Immunoprecipitation Learn more about immunoprecipitation using Dynabeads®, or follow the below links to the specific products: Kit with beads & buffers: |
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