FACT: Almost all background is removed with Dynabeads®
Simplicity is the key.

Remove all unwanted buffer, but don't disturb/lose your precious sample (animation at right).

Fast kinetics with Dynabeads® means fast protocols. And you'll avoid build-up of non-specific binding over time. And obviously, the recombinant protein A/G on the beads have no albumin binding sites.

No wonder researchers are moving to magnetic separation for their IP !

Compare IP methods (click to enlarge)
  Solution to two typical IP problems:

FACT: Preclearing is really not necessary with Dynabeads®

Still do preclearing thinking this will help reduce background?

With Dynabeads®, you get:
•  A larger surface area 
•  Faster binding kinetics
•  30 minute protocol time
•  A smooth bead-surface with a chemistry designed to avoid non-specific binding

No wonder researchers are moving to magnetic separation for their IP !

 
Skip redundant steps!
Dynabeads vs Sepharose for Immunoprecipitation
Four persons each performed two parallells using sepharose (incl. preclearing, SP1-SP4) and magnetic separation (without preclearing, DP1-DP4) . As seen, the yield and reproducibility is significantly better with Dynabeads®.

FACT: High capacity is not always a good thing

You want to balance capacity, yield, specificity, and cost.

An unbalanced outer/inner surface area (as seen e.g. with Sepharose® slurry) slows down the kinetics and increases the level of non-specific binding.

With Dynabeads®, you get:
•  Rapid binding kinetics
•  No inner surface to trap impurities
•  Effective and quick washing
•  Better results - faster

No wonder researchers are moving to magnetic separation for their IP !

 
Optimize the antigen binding capacity:
 
Immunoprecipitation vs Sepharose
The antigen (Ag) capacity is determined by the amount of available antibody (Ab). With Dynabeads®, all the Abs are bound on the surface. There's no excess interior surface, and hence no dead volume to trap Ab, Ag, or impurities.

FACT: You can save money using Dynabeads®

The right capacity and use of your Ab!

Slurry is typically considered to be cheap. Yet, the price per sample when using Dynabeads® is comparable, or even lower if pre-clearing is involved.

With Dynabeads® you'll: 
•  Optimize Ab capacity and usage
•  Maximize target protein recovery 
•  Cut protocol time to <30 min

Traditional IP techniques hasn't changed much over the last 25 years.

It's about time the old IP-myths are busted. Scientists are keen to try Dynabeads®. And they love what they see!
 
Don't get left behind. Bring your IP tools up to date now!

 
Cost Considerations:
 
Price per sample
As it is difficult to work with volumes lower than 50µl of 50% slurry,  the excess capacity cannot be fully utilized (see Myth 3).  Slurry is cheap. As shown, the price per sample when using Dynabeads® is comparable with 1-10 µg of Ab, and HALF THE PRICE if pre-clearing with Sepharose®/agarose is done.  

Choose Your Product:

This YouTube video will help you find the optimal product::

Interactive Selection Guide to Immunoprecipitation

Learn more about immunoprecipitation using Dynabeads®, or follow the below links to the specific products:

Kit with beads & buffers:
Immunoprecipitation Kit Protein A
Immunoprecipitation Kit Protein G 

Beads only:
Dynabeads® Protein A
Dynabeads® Protein G

Promo Code

But remember to get your PROMO CODE first!

 

Speed: 
30 minute IP protocol - in one tube!

Ease-of-use: 

  • No pre-treatment/dilution.
  • No centrifugation.
  • No columns


Extremely gentle: 
Bound only by affinity interactions, your target protein and protein complexes are preserved intact. The gentle Dynabeads® Protein A / Protein G ensure minimal physical stress, maximum sensitivity and yield, with no loss of target protein.

Did You See These YouTube Videos?

Sepharose® is a trademark of GE Healthcare companies.