Elution of the streptavidin - biotin bond

The interaction between streptavidin and biotin is formed very rapidly. Once formed, the interaction is unaffected by wide extremes of pH, temperature, organic solvents and other denaturing agents.

We have a Dynabeads Streptavidin TRIAL KIT with 1 ml of each bead-type, so you can try them out with your own application.
The table below describes some typical elution efficiencies of the biotin-streptavidin interaction under a variety of elution conditions. Please note that the elution efficiencies described are for free biotin, and might differ from that of a biotinylated specific ligand.

Temperature & Time Elution Solution Elution Efficiency
90°C for 10 minutes 10 mM EDTA pH 8.2 and 95% formamide
 96.8%
90°C for 5 minutes 10 mM EDTA pH 8.2 and 95% formamide 96.4%
90°C for 2 minutes 10 mM EDTA pH 8.2 and 95% formamide 96.8%
65°C for 5 minutes 10 mM EDTA pH 8.2 and 95% formamide 96.4%
65°C for 2 minutes10 mM EDTA pH 8.2 and 95% formamide
 97.9%
37°C for 10 minutes 10 mM EDTA pH 8.2 and 95% formamide 41.9%
90°C for 10 minutesH2O
 7.3%
90°C for 10 minutes10 mM EDTA pH 8.2
52.0%
90°C for 10 minutes95% formamide
 35.9%
90°C for 10 minutes30 mM NaOAc pH 9 and 95% formamide95.5%
90°C for 10 minutes80 mM NaOAc pH 9 and 95% formamide
 97.3%
90°C for 10 minutes140 mM NaOAc pH 9 and 95% formamide95.4%