Streptavidin-coupled Dynabeads®

Streptavidin-coupled Dynabeads®

Isolate any biomolecule – just add your biotinylated ligand.

Over the past 15 years, streptavidin-coupled Dynabeads® have been widely used and are cited in thousands of papers for diverse manual and automated applications. Dynabeads® are also employed on more than 25,000 routine IVD instruments worldwide. The portfolio of streptavidin-coupled Dynabeads® available is presented below.

Advantages using Dynabeads® Streptavidin

  • All you need is a tube and a magnet
  • No centrifugation, precipitation, or filtration
  • Robust and gentle, preserving protein nativity
  • Few handling steps and no sample dilution
  • Absolute consistency
  • Optimal binding kinetics and minimal sample loss enable quantitative isolations
  • All protocols can be easily automated


To allow flexibility, four different types of streptavidin-coupled Dynabeads® are available. Check out the Dynabeads Streptavidin SELECTION GUIDE further down the page to see the different characteristics.

There's also a Dynabeads Streptavidin TRIAL KIT with 1 ml of each bead-type, so you can try them out with your own application.

All bead-types are in principle applicable to the same type of applications. Your choice should be directed by the nature of your target and sample, the buffers and solutions applied. To obtain best possible results, choose the bead with the size, charge and degree of hydrophobiocity most appropriate for your application.

ProductCapacityCharacteristics and
Properties		Ideal for:
Dynabeads® M-280 Streptavidin Free biotin:
650 - 900 pmoles/mg beads.

Biotinylated Ig:
5-10 µg/mg beads.
  • Hydrophobic bead surface
  • Based on tosylactivated beads
    Diameter: 2.8 µm
  • Size distribution: CV < 3%
  • BSA as blocking protein
    Isoelectric point: pH 5.0
  • Low charge (-10 mV (at pH 7)
  • Iron content (Ferrites): 12% (17%)
  • Immunoassays/Immunodiagnostics
  • Purification of DNA/RNA binding proteins
  • Protein purification
  • Phage display
  • Biopanning
  • Cell isolation
     
Dynabeads® MyOne™ Streptavidin T1 Free biotin:
> 1,300 pmoles/mg beads.

Biotinylated Ig:
20 µg/mg beads
  • Hydrophobic bead surface
  • Based on tosylactivated beads
    Diameter: 1.05 µm
  • Size distribution: CV < 3%
    BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (-10 mV (at pH 7)
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Immunoassays/Immunodiagnostics
  • Purification of DNA/RNA binding proteins
  • Protein purification
  • Phage display
  • Biopanning
  • Cell isolation
  • Well suited for automated applications
Dynabeads®
M-270 Streptavidin  		Free biotin:
650 - 1,350 pmoles/mg beads.

Biotinylated Ig:
5-10 µg/mg beads.
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
  • Diameter: 2.8 µm
  • Size distribution: CV < 3%
  • No blocking proteins used
  • Isoelectric point: pH 4.5
  • Highly charged (-50 mV (at pH 7)
  • Iron content (Ferrites): 14% (20%)
  • Low aggregation of beads in high salt solutions
  • Sequence specific DNA/RNA capture in Nucleic Acid Diagnostics.
  • Use in protocols that require GTC lysis or high salt concentrations.
  • Preparation of single-stranded DNA.
  • Immunoassays/Immunodiagnostics with hydrophobic targets
Dynabeads® MyOne™ Streptavidin C1 Free biotin:
> 2,500 pmoles/mg beads

Biotinylated Ig:
15-20 µg/mg beads
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
    Diameter: 1.05 µm
  • Size distribution: CV < 3%
  • No blocking proteins used
  • Tween 20 in the buffer
  • Isoelectric point: pH 5.2
  • Medium charged (-35 mV (at pH 7))
    Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Low aggregation
  • Sequence specific DNA/RNA capture in Nucleic Acid diagnostics
  • Preparation of single-stranded DNA
  • High throughput nucleic acid clean up protocols
  • Sample preparation of proteins for mass spectrometry
  • Well suited for automated applications
Dynabeads® kilobaseBINDER™ Kit70 pmoles of a 4 kb biotinylated DNA fragment and 80 pmoles of a 10 kb fragment per mg beads
  • Contains Dynabeads® M-280 Streptavidin, a unique Binding Solution and a Washing Solution
  • Immobilization of long biotinylated DNA fragments (>2 kb)
  • Rapid, efficient and easy purification of nuclei from cytoplasm
  • Chromatin isolation within minutes
Dynabeads® Biotin BinderProcesses 2 x 10E9 cells (PBMC) or 200 ml whole blood
  • For use with your own standard biotinylated antobodies
  • Isolate cells from whole blood, buffy coat, PBMC, MNC or tissue digests
  • Depletion of one/multiple cell types from any sample from any species
  • Positive cell isolation for molecular downstream applications (without cell release)
CELLection® Biotin Binder Kit Processes 2 x 10E9 cells (PBMC) or 200 ml whole blood
  • For use with your own standard biotinylated antobodies
  • Streptavidin on the bead-surface is attached via a DNA linker, providing a cleavable site to release and emove the beads after isolation
  • Positive cell isolation and release
  • Isolate any cell from any sample from any species

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