Countess® Automated Cell CounterAutomated cell counting that is FAST, EASY and ACCURATE.
The Countess Automated Cell Counter can get your data in under 30 seconds without using a hemocytometer. |
| The Countess™ automated cell counter uses state-of-the-art optics and image analysis to automate cell counting. The Countess™ automated cell counter is a benchtop counter designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue technique. Using the same amount of sample that you currently use with the hemocytometer, the Countess automated cell counter takes 30 seconds per sample for a typical cell count and is compatible with a wide variety of eukaryotic cells and provides information on cell size. Just mix 10 μl of sample with 10 μl of trypan blue, and pipet into Countess® chamber slide. Insert the slide into the instrument, press the “Count Cells” button, and get your results displayed in 30 seconds! |
A single, sample measurement in 30 seconds provides the following data:
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The Countess™ automated cell counter is supplied with disposable Countess™ cell counting chamber slides that contain two enclosed chambers to hold the sample to allow you to measure two different samples or perform replicates of the same sample. The cell counting occurs in the central location of the counting chamber and the volume counted is 0.4 μL, the same as counting four (1 mm × 1 mm) squares in a standard hemocytometer.
Instrument Specifications
| Processing Time | 30 seconds |
| Cell Sample Range | 1 x 104 - 1 x 107 cells/ml |
| Optimal Cell Size | 50 µm to 60 µm |
| Instrument Type | Benchtop Cell Counter |
| Software | Countess Software |
| Countess USB Drive | 1 Gigabyte |
| Instrument Dimensions | 27 cm (w) x 20 cm (d) x 19 cm (h) |
| Weight | 2.1 kg (4.6 lbs.) |
Countess Cell Counting Chamber Slide Specifications
| Chamber Volume | 10 µL | |
| Material | Polymethylmethacrylate (PMMA) | |
| Dimensions | 75 mm (w) x 25 mm (d) x 1.8 mm (h) | |
| Chamber Depth | 100 µm |
Environmental Conditions
| Operating Power | 100-240 VAC, 1.5A |
| Frequency | 50/60 Hz |
| Electrical Input | 12 VDC, 1.0 A |
| Operating Temperature | 10-35C |
| Maximum Relative Humidity | 20-80% |
| Altitude | < 2000 m |
| Transient Category | Installment categories 11 |
| Pollution Degree | 2 |
Validated Stem Cell Lines
| Cell Name (click for pdf) | Cell Type | Cell size (µm in diameter) |
|---|---|---|
| ADSC | Human Fat | 16-17 µm |
| ESC | Mouse Embryonic stem cells | 10 µm |
| HESC | Human Embbryonic stem cells | 12-13 µm |
| NSC | Rat Fetal Neuronal Stem Cells | 11 µm |
Validated Cell Lines
| Cell Name (click for pdf) | Cell Type | Cell size (µm in diameter |
|---|---|---|
| A431 | Human Skin | 15.5 µm |
| ADSC | Human Fat | 16-17 µm |
| CHO-M1 | Hamster Ovary | na |
| CHSE | Fish Embryonic | 16 - 17 µm |
| COLO-205 | Human Intestine | na |
| COS-7 | Monkey Kidney | na |
| ESC | Mouse embryonic stem cells | 10 µm |
| HASMC | Human smooth muscle | 20 µm |
| HEK-293 | Human Kidney | 13 µm |
| HEKn | Human Epidermal Keratinocytes, neonstal | 12.5 µm |
| HepG2 | Human Liver | 18 µm |
| HESC | Human Embryonic Stem Cells | 12 - 13 µm |
| HL-60 | Human Blood | na |
| HPAEC | Human Pulmonary Artery Endothelial | 13 µm |
| HPASMC | Human Smooth Muscle | 20 µm |
| HUVEC | Human Umbilical Vein | 17 µm |
| J774(MMM) | Mouse Blood | 13-14 µm |
| Jurkat | Human Blood | 12 µm |
| K-562 | Human Bone/Marrow | na |
| MCF-7 | Human Mammary | 20-24 µm |
| MRC-5 | HUman Lung | 18 µm |
| NIH/3T3 | Mouse Embryonic | 18 µm |
| NSC | Rat Fetal Neuronal Stem Cells | 11 µm |
| PC-12 | Rat Adrenal | 12-14 µm |
| SF-21 | Insect Ovary | na |
| U-2 OS | Human Bone/Marrow | na |
| U266 | Human Blood | 12-13 µm |
![]() | Download:Instructions: |
Before Installing:
1. Save any data from csv file you wish to archive
a. Go to ‘Save’ menu
b. Press ‘View csv file’
c. Press ‘save to USB’
2. Start a new CSV file
a. Go to ‘Save’ screen
b. Press ‘Start new CSV’
c. When prompted, press ‘OK’ to erase previous data and start new csv file
Install Updates:
1. Download and save .exe file to your USB drive
2. Open .exe file and click ‘Extract’ (5 files will be unzipped onto your USB)
3. Insert USB drive into Countess USB port
4. Access ‘Settings’ screen and press ‘Update’
5. Press ‘OK’ after being prompted to update
6. When update is complete, you will be prompted to restart the instrument.
Calibration:
1. Press ‘Settings’ menu
2. Press ‘Calibrate’ button
3. Follow instructions on the calibration screen
4. Once complete, you will be prompted to restart the instrument
Update your PC Software:
In order to view the new Countess features on your computer, you will need to install the latest software update.
Note: The software is not required to run the instrument. Install the software only if you'd like to see the results on your computer and print a summary report.
Application Notes
Counting Clumpy Primary Cells
Using 0.1% Concentration of Trypan Blue
Protocol For Using Countess® with TrypLE™ Cell Dissociation Products
Flow Cytometry vs. the Countess® Cell Counter
Countess FAQs
Technical Notes
Comparison: Vi-CELL® XR Cell Viability Analyzer
Hemocytometer
Coulter Counter
Improving Downstream Results
Countess Application Posters
Countess® FASEB 2009 poster
Countess® ABRF 2009 poster
ASCB 2008 Countess® poster
Optimizing cell density in the FluxOR™ assay poster
Questions? Please contact us at techsupport@invitrogen.com or 1-800-955-6288












