ChargeSwitch® EasyPlex™ Multiplex PCR with Cystic Fibrosis Assay

Multiplex PCR performed directly on genomic DNA.
Genomic DNA was isolated from frozen blood using ChargeSwitch® beads (control) or the ChargeSwitch® EasyPlex™ gDNA Kit. An optimized primer mix for the CFTR gene (Tm Bioscience) and Invitrogen Platinum® Taq Polymerase were used to perform multiplex PCR directly in the wells of the coated plate. The amplified products were separated on a 4–12% gradient polyacrylamide gel, and stained with SYBR® Gold Nucleic Acid Gel Stain. Reactions performed in the wells of the ChargeSwitch® EasyPlex™ plate were of the same quality as reactions performed on ChargeSwitch® bead–purified template. M: 100bp DNA ladder, NTC: no-template control