ChargeSwitch® EasyPlex™ gDNA Kits
Spend Less Time Setting Up and More Time Analyzing Results
No beads, no membranes, no centrifugation, no vacuum manifold—not even a transfer between tubes or plates. Get your results faster than ever. With the ChargeSwitch® EasyPlex™ gDNA Kit’s simple protocol, you’ll save time by going directly from genomic DNA (gDNA) isolation to PCR in one well, in less than 45 minutes.
High-Performance gDNA Without Elution, Contamination, and False Positives
ChargeSwitch® kits come with aqueous buffers that do not contain guanidine or ethanol, which can inhibit downstream applications. Genomic DNA can be isolated from as little as 10 µL whole blood or 1–2 x 104 cells per well. Purified gDNA is amplified directly from microplate wells or 8-well strips, without elution, and is suitable for downstream procedures such as multiplex PCR, qPCR, STR (short tandem repeat) analysis, WGA (whole genome amplification), and sequencing. In addition, ChargeSwitch® EasyPlex™ gDNA Kits are compatible with most automated liquid handling workstations, and contain semi-skirted microplates and 8-well strips that fit into most thermal- and real-time- cyclers for high-throughput procedures (Figures 1 and 2). With the ChargeSwitch® EasyPlex™ gDNA Kit, you get successful, highly-pure gDNA results using less sample, contamination, sample error, and false positives.
Figure 2. ChargeSwitch® EasyPlex™ Multiplex PCR with Cystic Fibrosis Assay—performed directly on genomic DNA. Genomic DNA was isolated from frozen blood using ChargeSwitch® beads (control) or the ChargeSwitch® EasyPlex™ gDNA Kit. An optimized primer mix for the CFTR gene (Tm Bioscience) and Platinum® Taq Polymerase were used to perform multiplex PCR directly in the wells of the coated plate. The amplified products were separated on a 4–12% gradient polyacrylamide gel, and stained with SYBR® Gold Nucleic Acid Gel Stain. Reactions performed in the wells of the ChargeSwitch® EasyPlex™ plate were of the same quality as reactions performed on ChargeSwitch® bead–purified template. M: 100bp DNA ladder, NTC: no-template control.
The ChargeSwitch® EasyPlex™ gDNA Kit is easy to automate on most automated liquid handling work stations (Figure 3), without the need for any special equipment for processing (such as a vacuum manifold, shaker, or centrifuge). Depending on your deck and pipetting configuration, a single plate can take as little as 30 minutes to process, after sample lysis.
A Revolution in Viral Nucleic Acids Research, Specifically Designed for Small Samples (≤ 30 µL)
- Direct amplification from viral nucleic acids bound to a microtiter plate surface without any elution—obtain pure, undiluted viral nucleic acids
- Fast processing with minimal handling—from lysis to PCR within an hour
- Flexible automation—easy set-up on most automated liquid handling workstations without the need for vacuum manifolds, shakers, and centrifugation
Learn more about the ChargeSwitch® EasyPlex™ Viral RNA/DNA Kit