For the direct conjugate strategy, what would be the proper negative control?
In double- or triple-labeling experiments, is there any problem with incubating all of the primaries in the same step?
Is it possible to combine labeled primary antibodies with other same-species antibodies which would be secondary-labeled with different dyes in a multiplex protocol?
What kind of blocking reagent is good for fluorescent labeling?
Is there a special blocking agent used to block endogenous biotin?
Is there a special blocking agent used to block endogenous peroxidase when using TSA amplification?
Do you suggest using BSA or casein as a blocking solution?
How many dyes per avidin?
For secondary labeling, can sensitivity be increased by using higher concentrations of secondary antibody?
Does my secondary have to be from a species different from that of my tissue?
if you need to use chicken anti-goat and goat anti-rabbit secondaries, is it possible to prevent them from binding to one another?
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.