Fluorescent Labeling Techniques Covered by Molecular Probes® Scientists

Fluorescent probes enable researchers to detect particular components of complex biomolecular assemblies, such as live cells, with exquisite sensitivity and selectivity. In the Molecular Probes® fluorescent labeling webinars module, we cover the various methods and strategies for labeling biomolecules as well as the tools available to help you in your work.

Recorded Molecular Probes® Fluorescent Labeling Webinars

 

A Practical Approach to Antibody Labeling
Original Air Date: June 7, 2012

The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. This webinar will provide you with:

  • An overview of our antibody labeling kits
  • Guidance on which methods are ideal for specific applications and experiments
  • Tips and tricks to optimize your labeling protocol

View the recorded webinar

 

 

Fluorophore Selection in Experimental Design
Original Air Date: April 26, 2012

The choice of fluorophore is one of the first important decisions to make in developing an experiment.  Fluorophores are compounds that emit light when excited with another, lower, wavelength, but there are many different types.  This webinar will cover:

  • How to choose the best organic dye for an assay
  • Describe quantum dots and how they compare to other dyes
  • When to use a phycobiliprotein like R-PE or APC?
  • When to use fluorescent proteins, like GFP
  • Choosing a suitable dye to match your instrument

In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluors to help you choose and develop the best assay for your needs.

View the recorded webinar

 

 

A Comparison of Basic Immunofluorescent Labeling Strategies
Original Air Date: Thursday, February 16, 2012

In this webinar we compare different immunofluorescent labeling strategies, exploring the pros and cons of each method. Viewers learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We also present a simple decision tree to aid in determining the best method for each situation.

View the recorded webinar  View the FAQs

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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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