Nuclear Stains Protocol
|
Sample Image
NucRed™ Live 647 ReadyProbes™ reagent. hUVEC cells grown in a MatTek glass bottom dish overnight then stained with NucRed™ Live 647 ReadyProbes™ reagent. |
| ReadyProbes™ reagent | Storage | Filter | Ex/Em | Cat. No. |
|---|---|---|---|---|
| NucBlue® Live Cell Stain | Room Temperature (RT) (≤25°C) | DAPI | 358/461 | R37605 |
| NucBlue® Fixed Cell Stain | RT (≤25°C) | DAPI | 352/461 | R37606 |
| NucGreen™ Dead 488 Reagent | RT (≤25°C) | FITC | 504/523 | R37109 |
| Propidium Iodide Reagent | RT (≤25°C) | RFP | 535/617 | R37108 |
| NucRed™ Live 647 Cell Stain | RT (≤25°C) | Cy®5 | 638/686 | R37106 |
| NucRed™ Dead 647 Cell Stain | RT (≤25°C) | Cy®5 | 642/661 | R37113 |
Actin Stains Protocol
|
Sample Image
ActinGreen™ 488 ReadyProbes™ reagent. BPAE cells were fixed, permeabilized and blocked using the Image-iT® Fixation/Permeabilization Kit. Tubulin was labeled using an anti-tubulin antibody followed by detection with an Alexa Fluor® 594 secondary. Actin was stained using ActinGreen™ 488 ReadyProbes™ reagent and nuclei were stained with NucBlue® Fixed Cell Stain. |
| ReadyProbes™ reagent | Storage | Filter | Ex/Em | Cat. No. |
|---|---|---|---|---|
| ActinGreen™ 488 Reagent | Room Temperature (RT) (≤25°C) | FITC | 495/518 | R37110 |
| ActinRed™ 555 Reagent | RT (≤25°C) | RFP | 540/565 | R37112 |
Apoptosis Reagents Protocol
|
Sample Image
CellEvent® Caspase 3/7 Green ReadyProbes™ reagent. HeLa cells were loaded with CellEvent® Caspase 3/7 Green ReadyProbes™ reagent, then treated with 0.5 uM staurosporine or vehicle control for 4 hours. Untreated cells (A) have minimal fluorescence, while treated cells (B) have a significant increase in fluorescence (shown in green) indicating apoptosis. |
| ReadyProbes™ reagent | Storage | Filter | Ex/Em | Cat. No. |
|---|---|---|---|---|
| CellEvent® Caspase 3/7 Green | Room Temperature (RT) (≤25°C) | FITC | 495/518 | R37111 |
Imaging Accessories
 | U-2 OS Cells Fixed and Permeabilized with Image-iT® Fixation/Permeabilization Kit. U-2 OS cells expressing CellLight® Mitochondria-GFP were fixed and permeabilized with Image-iT® Fixation/Permeabilization Kit. Actin fibers were stained with Alexa Fluor® 594 phalloidin and nuclei with NucBlue® Live Cell Stain. |
| ReadyProbes™ reagent | Dropper | Storage | Live or Fixed | Cat. No. |
|---|---|---|---|---|
| BackDrop® Background Suppressor | Yes | Room Temperature (RT) (≤25°C) | Live | R37603 |
| Live Cell Imaging Solution | No | RT (≤25°C) | Live | A14291DJ |
| Image-iT® Fixation/Permeabilization Kit | No | RT (≤25°C) | Fixed | R37602 |
| Image-iT® FX Signal Enhancer | Yes | RT (≤25°C) | Fixed | R37107 |
| LIVE/DEAD® Cell Imaging Kit (488/570) | No | –20°C | Live | R37601 |
Citations
- Asakawa K et al. (2012) Hair organ regeneration via the bioengineered hair follicular unit transplantation. Scientific Reports doi:10.1038/srep00424.
- CB et al. (2012) A three-dimensional in vitro tumor platform for modeling therapeutic irreversible electroporation. Biophysical Journal 103(9): 2033-2042.
- Lee JM et al. (2012) Cbl-independent degradation of Met: ways to avoid agonism of bivalent Met-targeting antibody. Oncogene doi: 10.1038/onc.2012.551.
- McGrail DJ (2012) Biochemical analysis predicts the decreased human mesenchymal stem cell function before molecular differences. Exp Cell Res doi: 10.1016/j.yexcr.2012.11.017.
- Park MH et al. (2012) Complexation-induced biomimetic long range fibrous orientation in a rigid-flexible block copolymer thermogel. Adv Func Mat 22(24);5118-5125.
- Shikatani EA et al. (2012) Inhibition of proliferation, migration, and proteolysis contribute to corticosterone-mediated inhibition of angiogensis. PLoS One doi:10.1371/journal.pone.0046625.
- Toyoshima K et al. (2012) Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches. Nature Comm doi:10.1038/ncomms1784.
- Weng A et al. (2012) Saponins modulate the intracellular trafficking of protein toxins. Jour Contr Release doi: 10.1016/j.jconrel.2012.10.002.
