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SYTOX® Dead Cell Stains




Invitrogen offers Molecular Probes® cell-impermeant nucleic acid  SYTOX® Dead Cell Stains.  Because they do not cross intact cell membranes  and also increase fluorescence upon dsDNA binding they are among our most brilliant dead cell stains.  Easy to use, SYTOX® dead cell stains can be applied to cells and visualized without an additional wash step because they are non-fluorescent in aqueous media. These stains can be used on multiple platforms including fluorescence microscopy, flow cytometry, and microplates.

 
Dye Name
Excitation Source ExMax (nm)
EmMax (nm)
Emission Color
SYTOX® Blue dead cell stain "for flow cytometry"
UV, 405, 440
470480
SYTOX® Blue dead cell stainUV, 405, 440470480
SYTOX® Green dead cell stain488504523
SYTOX® Orange dead cell stain532
547570
SYTOX® Red dead cell stain633 or 635
640658
SYTOX® AADvanced™ dead cell stain488, 532
647 
     

SYTOX® Green Dead Cell Stain

SYTOX® Green dead cell stain has the highest nucleic acid affinity of all of the SYTOX® dead cell stains. Exhibiting a 100-fold increase in fluores- cence upon nucleic acid binding and minimal base selectivity (unlike DAPI and propidium iodide), SYTOX® Green dead cell stain is great for multi-parametric analysis with blue- or red- fluorescent labels.

SYTOX® Orange Dead Cell Stain

SYTOX® Orange dead cell stain binds nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide.

SYTOX® Red Dead Cell Stain

SYTOX® Red dead cell stain is a simple and quantitative single-step dead-cell indicator for use with red laser-equipped flow cytometers. SYTOX® Red dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with com- promised plasma membranes but will not cross uncompromised cell membranes.

SYTOX® Blue Dead Cell Stain

SYTOX® Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. SYTOX® Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes.

SYTOX® Dead Stain Cells in Action

 
Nuclear deformation of an apoptotic cell visualized with the SYTOX® Green dead cell stain.  Induced Jurkat cells stained with SYTOX® Green dead cell stain analyzed by flow cytometry. 
 
Micrococcus luteus stained with SYTOX® Blue dead cell stain.
 SYTOX® Blue dead cell stain using a mixture of heat-killed and untreated Jurkat cells.


HeLa cells labeled with anti–Golgin-97 antibody and detected using Alexa Fluor® 647 goat anti–mouse IgG antibody and SYTOX® Orange dead cell stain.
SYTOX®  Red dead cell stain using untreated and heat-treated human peripheral blood leucoytes.

Assays using SYTOX® Green Dead Cell Stain

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