SYTOX® Dead Cell Stains

 Invitrogen offers Molecular Probes® cell-impermeant nucleic acid  SYTOX® Dead Cell Stains.  Because they do not cross intact cell membranes  and also increase fluorescence upon dsDNA binding they are among our most brilliant dead cell stains.  Easy to use, SYTOX® dead cell stains can be applied to cells and visualized without an additional wash step because they are non-fluorescent in aqueous media. These stains can be used on multiple platforms including fluorescence microscopy, flow cytometry, and microplates.

View all SYTOX® Dead Cell Stain products

 
Dye NameExcitation SourceExMax (nm)EmMax (nm)Emission Color
SYTOX® Blue Dead Cell Stain, for flow cytometryUV, 405, 440470480 
SYTOX® Blue Nucleic Acid Cell StainUV, 405, 440470480
SYTOX® Green Dead Cell Stain, for flow cytometry488504523 
SYTOX® Green Nucleic Acid Stain488504523 
SYTOX® Orange Dead Cell Stain, for flow cytometry532547570 
SYTOX® Orange Nucleic Acid Stain532547570
SYTOX® Red Dead Cell Stain633 or 635640658
SYTOX® AADvanced™ Dead Cell Stain488, 532546 647
SYTOX® Dead Cell Stain Sampler KitAllAllAll
            

 

SYTOX® Green Stains

SYTOX® Green dead cell stain has the highest nucleic acid affinity of all of the SYTOX® dead cell stains. Exhibiting a 100-fold increase in fluores- cence upon nucleic acid binding and minimal base selectivity (unlike DAPI and propidium iodide), SYTOX® Green dead cell stain is great for multi-parametric analysis with blue- or red- fluorescent labels.

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SYTOX® Orange Nucleic Acid Stain

SYTOX® Orange dead cell stain binds nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide.

SYTOX® Red Dead Cell Stain

SYTOX® Red dead cell stain is a simple and quantitative single-step dead-cell indicator for use with red laser-equipped flow cytometers. SYTOX® Red dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with com- promised plasma membranes but will not cross uncompromised cell membranes.

SYTOX® Blue Stains

SYTOX® Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. SYTOX® Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes.

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SYTOX® Dead Stain Cells in Action

 
Nuclear deformation of an apoptotic cell visualized with the SYTOX® Green nucleic acid stain.
  
Induced Jurkat cells stained with SYTOX® Green nucleic acid stain analyzed by flow cytometry. 
     
 
Micrococcus luteus stained with SYTOX® Blue nucleic acid stain.
  
SYTOX® Blue dead cell stain using a mixture of heat-killed and untreated Jurkat cells.
     
 
HeLa cells labeled with anti–Golgin-97 antibody and detected using Alexa Fluor® 647 goat anti–mouse IgG antibody and SYTOX® Orange dead cell stain.
  
SYTOX®  Red dead cell stain using untreated and heat-treated human peripheral blood leucoytes.

Assays using SYTOX® Green Dead Cell Stain

We have built a variety of assays using the versatile SYTOX® Green dead cell stain including kits for apoptosis, cell viability and metabolism as well as for staining fixed cells for fluorescence imaging.

Apoptosis Kits


Viability Kit


Fixed Cell Staining Kit


Prepared Slide for Fluorescence Imaging

 

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