Qdot® Probe Manuals and Application Notes
NEW! Use of Qdot® Probe Primary Antibody Conjugates in Flow Cytometry
Provides protocols and tips for using Qdot® probes primary antibody conjugates in flow cytometry, including advice on sample preparation, use of fixatives and instrumentation setup.
Qdot® Conjugates Protocol Handbook
Provides protocols for Qdot® probe immunolabeling of antigens in cultured cells, formalin-fixed paraffin embedded sections, frozen tissue sections and Western blotting with secondary antibody conjugates.
Conjugation of Qdot® ITK™ Carboxyl Quantum Dots to Streptavidin
Provides a protocol for conjugating Qdot® ITK™ Carboxyl Quantum Dots to streptavidin and subsequent purification of conjugate from excess streptavidin.
Conjugation of Qdot® ITK™ Amino (PEG) Quantum Dots to Streptavidin
Provides a protocol for conjugating Qdot® ITK™ Amino (PEG) Quantum Dots to streptavidin and subsequent purification of conjugate from excess streptavidin.
Conjugation of Qdot® ITK™ Amino (PEG) Quantum Dots to Phalloidin
Provides a protocol for conjugating Qdot® ITK™ Amino (PEG) Quantum Dots to phalloidin and subsequent purification of conjugate from excess phalloidin.
Flocculation of Qdot® ITK™ Organic Quantum Dots from Decane
Provides the procedure for replacing decane solvent the organic quantum dots are shipped in with the solvent of choice.
Protocol describing the use of Qtracker™ non-targeted Quantum Dots for in vivo imaging of mice
Provides the procedure for injection of mice with non-targeted quantum dots and conditions for short- and long-term observation. A reference list is included.
Qdot® Streptavidin Conjugates User's Manual
Presents the structure of probes, their optical and spectral properties, biological activity and filter selection guidelines. Protocols for single and dual immunolabeling with biotinylated antibodies and subsequent detection with Qdot® streptavidin conjugates are presented, along with a troubleshooting guide and references section.
Qtracker® Cell Labeling Kit Protocol
Provides protocols for subculturing and labeling of cell types such as HeLa and U-118 with Qtracker quantum dots. Cell types other than those listed above can also be used.
Qdot® Antibody Conjugation Kit User's Manual
Provides protocol for conjugating Qdot® probes to 300 μg of antibody of IgG subclass when using the kit. The procedure is divided into four steps: quantum dot activation, antibody reduction, conjugation and quenching and conjugate purification. Spectral characteristics, filter selection guide, frequently asked questions and references sections are also included.
Qtracker® Cell Labeling Kit Protocol for Cells in Suspension
Provides a simple, easy to use protocol for labeling any cell type in suspension with Qtracker cell labeling quantum dots.
Labeling of cellular targets with Qdot® anti-Fluorescein Conjugates
Provides a protocol for immunostaining of adherent cells from culture with either primary or secondary fluorescein-antibody conjugate, followed by secondary or tertiary detection with Qdot® anti-fluorescein conjugate.
Qdot® Antibody Conjugation Kit Supplemental Information Guide
A number of frequently asked questions regarding the structure and use of Qdot® Antibody Conjugation kits.
In-Gel Western Analysis with Qdot® Western Blotting Kits
Provides the protocol based on standard polyacrylamide gel electrophoresis to perform multicolor in-gel Western analysis. The procedure also describes the analysis of a dilution series of the GST protein that can be applied to other proteins.
Qdot® Western Blotting Kit User Manual
Provides the protocol for blocking and developing the mini-blots following electroblotting with Qdot® secondary antibody conjugates. A guide to image capture and analysis is included, along with a list of equipment compatible with the Probes.
Labeling Mouse Kidney Sections with Qdot® Wheat Germ Agglutinin Conjugate
Provides a protocol for staining of α-mannopyranosyl and α-glucopyranosyl residues in mouse kidney sections.
Labeling HeLa Cells with Qdot® Wheat Germ Agglutinin Conjugate
Provides a protocol for staining of α-mannopyranosyl and α-glucopyranosyl residues in cultured HeLa cells.