Qdot® Nanocrystal Recommended Filters
Recommended filters for use with Qdot® nanocrystals
| Omega Optical Filter Sets | Chroma Technology Filter Sets | |||
|---|---|---|---|---|
| Color | Optimal Filter Sets | Useable Filter Sets * | Optimal Filter Sets | Useable Filter Sets * |
| 800† | XF307 Qdot® 800 filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 800WB80 | XF308 Qdot® 800 filter set for multiplexing Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 840WB80 | Qdot® 800 filter set (30 nm EM; 32020) (460SPUV/475DCXRU /D800/30nm) Qdot® 800 filter set (50 nm EM; 32021) (460SPUV/475DCXRU /D800/50nm) | Cy7 (41009) Li-Cor for IRDye 800 (41037) Cy7 (SP106) |
| 705† | XF306 Qdot® 705 filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 710AF40 | XF140-2 XF70 XF110-2 XF141-2 XF48-2 | Qdot® 705 filter set (20 nm EM; 32014) (460SPUV/475DCXRU/ D705/20nm) Qdot® 705 filter set (40 nm EM; 32015) (460SPUV/475DCXRU/ D705/40nm) | Cy5 longpass (41024) Cy5 (41008) Cy5 narrow excitation (41033) Cy5.5 (41023) Alexa Fluor® 680 (41042) Cy5.5 (red-shifted; 41022) |
| 655 | XF305 Qdot® 655 filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 655WB20 | XF102-2 XF40-2 XF42 XF45 | Qdot® 655 filter set (20 nm EM; 32011) (460SPUV/475DCXRU/ D655/20nm) Qdot® 655 filter set (40 nm EM; 32012) (460SPUV/475DCXRU/ D655/40nm) | Texas Red® (41004) Propidium iodide (41005) Fura Red™ (31012) Chlorophyll (31017) Allophycocyanin (31006) |
| 605 | XF304 Qdot® 605 filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 605WB20 | XF108-2 XF102-2 XF103-2 | Qdot® 605 filter set (20 nm EM; 32003) (460SPUV/475DCXRU/ D605/20nm) Qdot® 605 filter set (40 nm EM; 32007) (460SPUV/475DCXRU/ D605/40nm) | Cy3 narrow excitation (41007a) Texas Red®/Cy3.5 (31004) TRITC (41002, 41002a, 41002b) Ethidium bromide (41006) |
| 585 | XF303 Qdot® 585 filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 585WB20 | XF101-2 XF137-2 XF152-2 | Qdot® 585 filter set (20 nm EM; 32004) (460SPUV/475DCXRU/ D585/20nm) Qdot® 585 filter set (40 nm EM; 32008) (460SPUV/475DCXRU/ D585/40nm) | R-PE (41003) Rhodamine LP (41032) FITC/PI (41016) |
| 565 | XF302 Qdot® 565 filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 565WB20 | XF104-2 XF105-2 | Qdot® 565 filter set (20 nm EM; 32005) (460SPUV/475DCXRU/ D565/20nm) Qdot® 565 filter set (40 nm EM; 32009) (460SPUV/475DCXRU/ D565/40nm) | Eosin (41011) Cascade Yellow™ (31038) JP2 (YGFP with EGFP; 31040) Auramine (31015) |
| 525 | XF301 Qdot® 525 filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitter: 525WB20 | XF100-3 XF100-2 XF115-2 XF89-2 | Qdot® 525 filter set (20 nm EM; 32006) (460SPUV/475DCXRU/ D525/20nm) Qdot® 525 filter set (40 nm EM; 32010) (460SPUV/475DCXRU/ D525/40nm) | FITC/RSGFP/Bodipy®/ Fluo-3/DiO (41001) FITC/RSGFP longpass (40012) BFP to GFP FRET (31032) BFP to GFP FRET wide excitation (31034) GFP wide blue excitation (31054) |
| All colors ‡ | XF300 Qdot® filter set Exciter 1: 425DF45 or Exciter 2: 415WB100 Dichroic: 475DCLP Emitters: 800WB80, 840WB80, 710AF40, 655WB20, 605WB20, 585WB20, 565WB20, 525WB20 | XF129-2 XF130-2 | Qdot® Multiple Emission Set (71014) (460SPUV, 475DCXRU, D525/20nm, D605/20nm, D565/20nm, D585/20nm) | UV (11000V2) Blue/Violet (11003V2) UV/Violet (11011V2) |
