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ProLong® Gold Antifade Reagent

The ProLong® Gold antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. For added convenience, ProLong® Gold antifade reagents containing the blue-fluorescent nuclear counterstain DAPI are also available, eliminating the need for a separate counterstaining step.
Outperform the competition across the spectrum
ProLong® Gold antifade reagent outperforms all other commercially available antifade reagents and causes little or no quenching of the initial fluorescent signal.

Compatible with the broadest range of dyes
ProLong® Gold antifade reagents offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.

Stabilize sample signals for months
ProLong® Gold antifade reagents cure within 24 hours, forming a semi-rigid gel. Mounted samples can be saved for months with appropriate storage.

ProLong® Gold Antifade Reagent in Action


Figure 1—A 20-second time series showing the enhanced resistance to photobleaching afforded by ProLong® Gold antifade reagent. Fixed bovine pulmonary artery endothelial cells (BPAEC) were labeled with anti–alpha-tubulin antibody (A11126) and visualized with fluorescein goat anti–mouse IgG antibody (F2761). The samples were mounted in ProLong® Gold antifade reagent (P36930, top) or phosphate-buffered saline (bottom). Images were acquired at 5-second intervals using a 40x/1.3 NA oil immersion objective with continuous illumination from a standard 100-watt Hg-arc lamp.
Figure 2—ProLong® Gold antifade reagent outperforms competitors’ antifades across the visible spectrum. While competitors’ reagents preserve signal strength at only specific wavelengths, ProLong® Gold antifade reagent offers stability across the entire spectrum.

  • Degree of photobleaching protection by ProLong® Gold antifade reagent for different fluorescent dyes.

    Fluorescent dye              Resistance of dye to photobleaching*
    Alexa Fluor® 350
    65%
    Alexa Fluor® 488
    85%
    Alexa Fluor® 546
    67%
    Alexa Fluor® 555
    80%
    Alexa Fluor® 568
    93%
    Alexa Fluor® 594
    92%
    Alexa Fluor® 633
    99%
    Alexa Fluor® 635
    100%
    Alexa Fluor® 647
    100%
    Alexa Fluor® 660
    94%
    Alexa Fluor® 680
    96%
    7-Aminoactinomycin D
    92%
    Cy™2
    89%
    Cy™3
    83%
    Cy™5
    100%
    Fluorescein
    85%
    BODIPY® FL
    54%
    Tetramethylrhodamine
    98%
    Texas Red®
    61%
    DAPI
    67%
    SYTOX® Green
    85%
    TO-PRO®-3
    85%
    *Expressed as percentage of initial fluorescence intensity remaining following 30 seconds of continuous illumination using a 40×/1.4NA objective and a 100-watt Hg-arc lamp as the light source.

Looking for our Original Prolong® Kit.

For performance and convenience we recommend you try our Prolong® Gold antifade reagent but if you still desire to learn more about our original Prolong® Antifade Kit follow the link below.