ProLong® Gold Antifade Reagent
 | The ProLong® Gold antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. For added convenience, ProLong® Gold antifade reagents containing the blue-fluorescent nuclear counterstain DAPI are also available, eliminating the need for a separate counterstaining step. |
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Outperforms The Competition
ProLong® Gold antifade reagent outperforms all other commercially available antifade reagents and causes little or no quenching of the initial fluorescent signal.
Stabilize Sample Signals Longer
ProLong® Gold antifade reagents cure within 24 hours, forming a semi-rigid gel. Mounted samples can be saved for months with appropriate storage.
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Compatible With Many Dyes
ProLong® Gold antifade reagents offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.
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Detailed Protocols using ProLong® Gold Antifade Reagent
ProLong® Gold Antifade Reagent in Action
A 20-second time series showing the enhanced resistance to photobleaching afforded by ProLong® Gold antifade reagent. Fixed bovine pulmonary artery endothelial cells (BPAEC) were labeled with anti–alpha-tubulin antibody (A11126) and visualized with fluorescein goat anti–mouse IgG antibody (F2761). The samples were mounted in ProLong® Gold antifade reagent (P36930, top) or phosphate-buffered saline (bottom). Images were acquired at 5-second intervals using a 40x/1.3 NA oil immersion objective with continuous illumination from a standard 100-watt Hg-arc lamp.
 | ProLong® Gold Antifade Reagent outperforms competitors’ antifades across the visible spectrum. While competitors’ reagents preserve signal strength at only specific wavelengths, ProLong® Gold reagent offers stability across the entire spectrum. AF = Alexa Fluor®. |
| Fluorescent dye | Resistance of dye to photobleaching* |
|---|---|
| Alexa Fluor® 350 | 65% |
| Alexa Fluor® 488 | 85% |
| Alexa Fluor® 546 | 67% |
| Alexa Fluor® 555 | 80% |
| Alexa Fluor® 568 | 93% |
| Alexa Fluor® 594 | 92% |
| Alexa Fluor® 633 | 99% |
| Alexa Fluor® 635 | 100% |
| Alexa Fluor® 647 | 100% |
| Alexa Fluor® 660 | 94% |
| Alexa Fluor® 680 | 96% |
| 7-Aminoactinomycin D | 92% |
| Cy™2 | 89% |
| Cy™3 | 83% |
| Cy™5 | 100% |
| Fluorescein | 85% |
| BODIPY® FL | 54% |
| Tetramethylrhodamine | 98% |
| Texas Red® | 61% |
| DAPI | 67% |
| SYTOX® Green | 85% |
| TO-PRO®-3 | 85% |
