Label. Detect. Share and Learn.   Share and learn with your colleagues with the Handbook Club on Facebook and the Molecular Probes Technology Network. Handbook Club On Facebook Molecular Probes® Tech Network		Label. Detect. Explore. Get more out of your experiments with Molecular Probes® products, tools, and educational content. Explore Molecular Probes® Products		Label. Detect. Design and Find. Design and find with the most complete fluorescent labeling and detection reference available. Order the Print Handbook Explore the Online Handbook

Tech Tips Two-photon absorption Doing two-photon microscopy? Here is a good link for two-photon absorption cross-sections for dyes: http://www.drbio.cornell.edu/cross_sections.html Antigen Retrieval Techniques Antigen retrieval techniques (used to unmask antigens in samples for immunolabeling) typically involve heating the sample but the best instrument, buffer, and timing differ from antigen to antigen. Here is one guide to different techniques: http://www.ihcworld.com/epitope-retrieval. Note: not all antigens require antigen retrieval. See All Molecular Probes® Tech Tips The History of Molecular Probes® Label. Detect. Take a trip down memory lane. Since 1975, Molecular Probes® reagents have been used to help address a wide variety of research questions and enabled cell biology researchers to accelerate their discoveries. Read More About the History of Molecular Probes		See Molecular Probes® In Action   Actin and Tubulin During Mitosis U20S cells transduced with Cellular Lights™ Actin-RFP and CellLight® MAP4-GFP. Images taken every 15 min for 16 hours. Endosomal Transport Along Microtubules U20S cells transduced with CellLight® MAP4-GFP and CellLight ® Early Endosome-GFP. Images taken every min for 30 minutes. Plasma Membrane Ruffling U20S cells transduced with Cellular Lights™ Actin-RFP and CellLights® Plasma Membrane-GFP and imaged the next day. Images taken every 3 secs for 5 mins. Michondrial Dynamics Through Cell Division U20S cells were transduced with CellLight® Mito-RFP and imaged every 5 mins for 16 hours. See more Cell Videos on the Molecular Probes® YouTube Channel Make Your Own Moments With CellLight® Reagents