CyQUANT® Cell Proliferation Assays
 | Count on CyQUANT® Cell Proliferation Assays when you need an accurate determination of cell number. Because cell DNA content is constant, measuring cell number using DNA provides an accurate indication of relative cell number. CyQUANT® Cell Proliferation Assays are ideal for high throughput screening, are more sensitive than colorimetric-based assays, and are not radioactive. Quick & easy, both kits have no washes, extractions, growth medium changes or long incubations. Neither rely on metabolic status of the cell.
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Pick Your CyQuant® Kit
CyQUANT® Cell Proliferation Assays
- For multi-day tests or when you need precision
- Samples are run, frozen, then assayed all at the same time using the same set of standards
- Freezing ensures precision
- Linear from 50 to 50,000 cells
- Up to 250,000 cells with increased dye concentration
- When you need real time data
- Streamlined – 1 hour start to finish
- No freeze step required
- Linear from 100-20,000 cells
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CyQUANT®  Quantitation of NIH 3T3 fibroblasts using the CyQUANT® Cell Proliferation Assay Kit. Fluorescence measurements were made using a microplate reader with excitation at 485 nm and emission detection at 530 nm. The linear range of the assay under these conditions is from 50 to 50,000 cells per 200 µL sample. The inset shows the linearity that can be obtained at very low number of cells. |
CyQUANT® NF  Quantitation of CHO (M1WT3) cells using the CyQUANT® NF Cell Proliferation Assay Kit. Fluorescence measurements were made using a microplate reader with excitation at 485 nm and emission detection at 530 nm. The linear range of the assay under these conditions is from 20 to 20,000 cells per 100 µL sample. The inset shows the linearity that can be obtained at very low number of cells. |
CyQUANT® Cell Proliferation Assays Kit Comparison
| CyQUANT® | CyQUANT® NF | |
|---|---|---|
| Linearity (# of cells) | 50-50,000 | 20-20,000 |
| Length of Assay | 3 hrs minimum | 1 hr |
| Optimal Assay Design | Multi-day or endpoint experiment | Real time data analysis |
| Freezing Step Required? | Yes (view protocol schematics) | No (view protocol schematics) |
| Sample Volume | 200 µl | 100 µl |
| Ex/Em | 480/520 nm | 497/520 nm |
| Validated Cell Lines | Mouse fibroblasts (NIH 3T3, CREBAG 2 cells), normal human umbilical endothelial cells (HUVEC), canine kidney cells (MDCK), chinook salmon embryo cells (RBL, rat glioma cells (C6) and mouse myeloma cells | HEK 293, HeLa, HepG2, 3T3 and CHO cells |
| detailed protocol | detailed protocol | |
| view references | view references | |
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