Click-iT® EdU Cell Proliferation Assays
The Click-iT® EdU cell proliferation assay utilizes the power of click chemistry to provide a superior alternative to traditional methods for detecting and quantitating newly synthesized DNA. Click-iT® assays use a modified nucleoside, EdU (5-ethynyl-2´-deoxyuridine), that is incorporated during DNA synthesis in a quick click chemistry reaction. | | - Superior alternative to BrdU
- No DNA denaturation required
- Streamlined click chemistry detection protocol for faster results
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Better Signal, Faster Results
Comparison of EdU and BrdU Signal Brightness
Sectioned formaldehyde-fixed, paraffin-embedded tissue was prepared from rats injected intraperitoneally with EdU (Right panel) or BrdU (Left panel). Proliferating cells within the intestinal villi that incorporated the nucleoside are pink. Nuclei in both samples were counterstained with Hoechst 33342 (gray). Images were acquired with a 20x objective on a Nikon Eclipse 800 epifluorescence microscope
Right Panel: EdU was detected in less than 4 hours using the reagents in the Click-iT® EdU Alexa Fluor® 647 Imaging Kit, with a 5 msec exposure time for image acquisition.
Left Panel: BrdU-labeled tissue was denatured with HCl, neutralized, and blocked prior to incubating with mouse anti-BrdU antibody overnight. After washing in blocking buffer, the tissue was incubated with goat anti-mouse Alexa Fluor® 647 conjugate. Images were acquired with a 2,000 msec exposure time.
Replace Your Cumbersome BrdU Assays
 | | Click-iT® EdU – click chemistry simplicity makes it the faster, friendlier alternative to 3H-thymidine and BrdU
Unlike assays using bromodeoxyuridine (BrdU), Click-iT® EdU assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, Click-iT® EdU utilizes click chemistry for detection in a variety of Alexa Fluor® dye fluorescent readouts. Furthermore, the streamlined detection protocol both reduces the total number of steps and significantly decreases the total amount of time.
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Find Your Click-iT® Detection Assay
With its equally small “footprint,” the Click-iT® detection molecule can easily penetrate complex samples for in-depth analysis of key biological processes, including assays for:
Flow Cytometry
 | | Click-iT® EdU Flow Cytometry Assay Kits use click chemistry to completely eliminate the DNA denaturation step required by BrdU assays (typically HCl, heat or digestion with DNase) – steps that not only disrupt dsDNA integrity which can affect nuclear counterstaining, but also potentially destroys cell morphology and antigen recognition sites. | | Click-iT® EdU Flow Assays
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Imaging
 | | The Click-iT® EdU Imaging Assays detect newly synthesized DNA without the need for antibodies (and long antibody incubations) with a simple click chemistry detection procedure that is complete within 30 minutes and multiplex-compatible for content and context-rich results. | | Click-iT® EdU Imaging Assays |
High Content Screening (HCS)
 | | By using click chemistry, Click-iT® EdU HCS assays have fewer steps and require less time to detect and quantitate newly synthesized DNA compared to traditional BrdU methods. The Click-iT® click chemistry assay is fully compatible with DNA staining, including dyes for cell cycle analysis and can also be multiplexed with surface and intracellular marker detection using antibodies.
| | 2 Plate HCS Kits10 Plate HCS Kits |
High Throughput Screening (HTS)
 | | The Click-iT® EdU Microplate Assay incorporates click chemistry for detection to provide a simple and rapid workflow with reduced wash steps provides a 25%+ time-savings advantage over traditional BrdU colorimetric or fluorescent cell proliferation assays. This assay uses the very sensitive and versatile red-fluorescent Amplex® UltraRed reagent for the readout which can be detected by either fluorescence or absorbance.
| | Microplate Assays |
Whole Animal
 | | The thymidine analog, EdU (5-ethynyl-2’- deoxyuridine) greatly simplifies the workflow when detecting cell proliferation in tissue sections. From start to finish, our optimized click chemistry assay for EdU detection is complete in as little as 90 minutes compared to the antibody-based BrdU method which takes 6-24 hours to complete and often requires signal amplification for a suitable signal.
| | EdU Products |
A20012,A20013,C10338,C10352,C10353,C10337,C10339,C10340,C10350,C10354,C10356,C10351,C10355,C10357,C10214,A10044,E10187,E10415,C10418,C10419,C10420,C10424,C10425
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