Amplex® Red Assays Selection Guide

Amplex Red® Enzyme Assays have been developed for a wide range of research areas, including:

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Cell Signaling and Lipids

Enzyme/Analyte Amplex® Red Assay Kit  Enzyme Cascade
Phospholipase C Phosphatidylcholine-Specific
Phospholipase C Assay (A12218)
 1) phosphatidyl substrate  phosphocholine + diacylglycerol →(PC-PLC)
2) phosphocholine → choline (alkaline phosphatase)
3) choline + O2 → betaine + H2O2 (choline oxidase)
4) H2O2 + Amplex® Red → resorufin (HRP)
Phospholipase D  Phospholipase-D Assay (A12219) 1) phosphatidyl substrate → choline + phosphatidic acid (PL-D)
2) choline + O2 → betaine + H2O2 (choline oxidase)
3) H2O2 + Amplex® Red → resorufin (HRP)
Cholesterol Oxidase
 Cholesterol (A12216) 1) Cholesterol esters → cholesterol + fatty acids (Cholesterol esterase)
2)  Cholesterol +O2 → Cholest-4-ene-3-one + H2O2 (Cholesterol oxidase)
3)  H2O2 + Amplex® Red → resorufin (HRP)
Sphingomyelinase
 Spingomyelinase (A12220) 1) Sphingomyelin → phosphorylcholine + ceramide (sphingomyelinase)
2) phosphorylcholine → choline (alkaline phosphatase)
3) choline + O2 → betaine + H2O2 (choline oxidase)
4) H2O2 + Amplex® Red → resorufin (HRP)
Inorganic Phosphate
PiPer™ Phosphate (P22061)
1) Pi + maltose → glucose-1-phosphate + glucose (maltose phosphorylase)
2) Glucose + O2 → gluconolactone + H2O2 (Glucose oxidase)
3) H2O2 + Amplex® Red → resorufin (HRP)
Inorganic Pyrophosphate
PiPer™ Pyrophosphate (P22062)
1) PPi → Pi (inorganic pyrophosphatase)
2) Pi + maltose → glucose-1-phosphate + glucose (maltose phosphorylase)
3) Glucose + O2 → gluconolactone + H2O2 (Glucose oxidase)
4) H2O2 + Amplex® Red → resorufin (HRP)
Amplex® Red Phosphatidylcholine-Specific phospholipase C Assay   Phospholipase C
Amplex® Red Phosphatidylcholine-Specific phospholipase C Assay (A12218). Detection of PC-PLC using the Amplex® Red reagent–based assay. Each reaction contained Amplex® Red reagent,  HRP,  alkaline phosphatase,  choline oxidase,  lecithin and the indicated amount of B. cereus PC-PLC.		Amplex® Red Phospholipase-D Assay   Phospholipase D
Amplex® Red Phospholipase-D Assay (A12219).  Detection of phospholipase D using the Amplex Red reagent–based assay.  Each reaction contained Amplex Red reagent,
HRP, choline oxidase, lecithin and the indicated amount of Streptomyces chromofuscus phospholipase D.
           
Amplex® Red Cholesterol and Cholesterol Oxidase Assay   Cholesterol/Cholesterol Oxidase
Amplex® Red Cholesterol and Cholesterol Oxidase Assay (A12216). Detection of cholesterol using the Amplex® Red reagent–based assay. Each reaction contained Amplex® Red reagent, HRP, cholesterol oxidase, cholesterol esterase, and the indicated amount of the cholesterol.		Amplex® Red Spingomyelinase Assay   Sphingomyelinase
Amplex® Red Spingomyelinase Assay (A12220).  Detection of sphingomyelinase using the Amplex® Red reagent–based assay. Each reaction contained Amplex® Red reagent,  HRP, choline oxidase, alkaline phosphatase, sphingomyelin, and the indicated amount of Bacillus cereus sphingomyelinase.
           
PiPer™ Phosphate Assy Kit   Inorganic Phosphate
PiPer™ Phosphate Assy Kit (P22061). The PiPer™ Phosphate Assay Kit provides an ultrasensitive fluorometric method for detecting as little as 0.2 µM Pi in purified enzyme systems with a 100 µL assay volume.		PiPer™ Pyrophospate Assay Kit   Inorganic Pyrophosphate
PiPer™ Pyrophospate Assay Kit (P22062). The PiPer™ Pyrophosphate Assay Kit provides a sensitive fluorogenic or chromogenic method for detecting as little as 0.4 µM (fluorescence) or 2 µM (absorbance) inorganic pyrophosphate in a 100 µL assay volume.

Neurobiology

Enzyme/AnalyteAmplex® Red Assay Kit Enzyme Cascade
Glutamate Oxidase
Glutamic acid/Glutamate Oxidase (A12221)1) L-glutamic acid + O2 → α-ketoglutarate + NH3 + H2O2  (glutamate oxidase)
2) H2O2 + Amplex® Red → resorufin (HRP)
Acetylcholinesterase
Acetylcholine/
Acetylcholinesterase (A12217)		1) acetylcholine → choline + acetate (Acetylcholinesterase)   
2) choline + O2 → betaine + H2O2 (choline oxidase)
3) H2O2 + Amplex® Red → resorufin (HRP)
Monoamine Oxidase (MAO) or Plasma Amine Oxidase (SSAO)Monoamine Oxidase (A12214)1) amine substrate + O2 → H2O2 (monoamine (MAO) or plasma amine oxidase (SSAO))
2) H2O2 + Amplex® Red → resorufin (HRP)
Amplex® Red Glutamic Acid  Glutamate Oxidase
Amplex® Red Glutamic Acid (A12221). Detection of l-glutamate oxidase using the Amplex® Red reagent-based assay. Each reaction contained Amplex® Red reagent, HRP, l-glutamate-pyruvate transaminase, l-glutamic acid, l-alanine, and the indicated amount of Streptomyces l-glutamate oxidase.		   Amplex® Red ACH/ACHE  Acetylcholinesterase
Amplex® Red ACH/ACHE (A12217). Detection of electric eel acetylcholinesterase activity using the Amplex® Red reagent–based assay. Each reaction contained acetylcholine, Amplex® Red reagent, HRP, choline oxidase, and the indicated amount of acetylcholinesterase.
          
Amplex® Red Monoamine Oxidase Monoamine Oxidase
Amplex® Red Monoamine Oxidase (A12214). Detection of plasma amine oxidase (an SSAO) activity using the Amplex® Red Monoamine Oxidase Assay Kit and benzylamine as the substrate. Each reaction contained benzylamine, HRP, Amplex® Red reagent, and the indicated amount of SSAO.		    

Inflammation and Immune Function

Enzyme/AnalyteAmplex® Red Assay Kit Enzyme Cascade
Neuraminidase (Sialidase)Neuraminidase (A22178)
1) terminal sialic acid → desialiated galactose  (neuraminidase)
2) desialiated galactose  +O2 → galactoaldehyde + H2O2 (galactose oxidase)
3) H2O2 + Amplex® Red → resorufin (HRP)
CatalaseCatalase (A22180)1) H2O2 → O2 + H2O (catalase)
2) H2O2 + Amplex® Red → resorufin (HRP)
Xanthine Oxidase
Xanthine/ Xanthine Oxidase Assay (A22182)1) hypoxanthine or xanthine + O2  → uric acid + superoxide (xanthine oxidase)
2) superoxide → H2O2 (spontaneously degrades)
3) H2O2 + Amplex® Red → resorufin (HRP)
Peroxidases (General)
Hydrogen Peroxide (A22188)1) H2O2 + Amplex® Red → resorufin (HRP)
MyeloperoxidaseMyeloperoxidase (E33856)
1) H2O2 + Amplex® UltraRed →Amplex® UltroxRed product (myeloperoxidase)
Amplex® Red Neuraminidase Kit   Neuraminidase (Sialidase)
Detection of neuraminidase activity in serum using the Amplex® Red Neuraminidase Kit (A22178).		Amplex® Red Catalase Assay Kit   Catalase
Detection of catalase using the Amplex® Red Catalase Assay Kit (A22180).
           
Amplex® Red Xanthine Oxidase Kit   Xanthine Oxidase
Detection of xanthine oxidase using the Amplex® Red Xanthine Oxidase Kit (A22182).		Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit   Peroxidases (General)
Detection of HRP using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (A22188).
       
EnzChek® Myeloperoxidase Kit   Myeloperoxidase
The EnzChek® Myeloperoxidase Kit (with Amplex® UltraRed) (E33856) provides a comprehensive set of components for accurate and sensitive quantitation of MPO in a variety of biological samples, including human serum.		 

Metabolism

Enzyme/AnalyteAmplex® Red Assay Kit Enzyme Cascade
UricaseUric Acid/Uricase (A22181)1) uric acid → allantoin + CO2 + H2O2 (uricase)
2) H2O2 + Amplex® Red → resorufin (HRP)
Glucose OxidaseGlucose/Glucose Oxidase (A22189)1) Glucose + O2 → gluconolactone + H2O2 (Glucose oxidase)   
2)  H2O2 + Amplex® Red → resorufin (HRP)
Galactose Oxidase
Galactose/Galactose Oxidase (A22179)
1) galactose + O2 → galacteraldehyde + H2O2 (Galactose oxidase)
2) H2O2 + Amplex® Red → resorufin (HRP)
Amplex® Red Uricase/Uric Acid Kit   Uricase
Detection of uricase using the Amplex® Red Uricase/Uric Acid Kit (A22181).		  Glucose/Glucose Oxidase Assay Kit   Glucose Oxidase
Detection of glucose oxidase using the Amplex® Red Glucose/Glucose Oxidase Assay Kit (A22189).
            
Amplex® Red Galactose/Galactose Oxidase   Galactose Oxidase
Amplex® Red Galactose/Galactose Oxidase (A22179). Detection of galactose oxidase activity using the Amplex® Red Galactose/Galactose Oxidase Assay Kit. Each reaction contained Amplex® Red reagent, HRP, galactose, and the indicated amount of galactose oxidase.		       
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