Amplex® Red Enzyme Assays
Invitrogen offers validated solutions for enzyme activity assays using Molecular Probes® extremely versatile Amplex® Red reagent (and the second generation Amplex® UltraRed reagent). This highly stable and sensitive fluorogenic substrate for horseradish peroxidase has been used to develop a variety of novel fluorogenic and chromogenic assays for enzymes that produce hydrogen peroxide. Additionally, these coupled enzyme assays permit the ultrasensitive quantitation of a diverse assortment of analytes including glucose, galactose, cholesterol, and hydrogen peroxide.
Amplex® Red Technology
How Amplex® Red Reagent Works
Amplex® UltraRed Reagent
Customize Your Amplex® Red Assay
- Extended dynamic range and sensitivity compared to classic colorimetric oxidase assays
- Fluorescence emission outside the range of compound autofluorescence
Quantitative enzyme activity detection
High Throughput compatibility
- Antibody-free detection of analytes at pennies per well
- Scalable assay formats - custom packaging available
Ability to customize enzyme assays to fit your needs
Each kit comes complete with all the reagents needed to detect the enzyme and analytes in common biological samples, with standards included for precise quantitation.
Amplex® Red Assays for Multiple Research Areas
Cell Signaling and Lipids
Lipases and inorganic phosphates are critical in many signaling pathways, from mTOR to GPCRs where they are involved in disease states ranging from cardiovascular to inflammation and cancer.
Inflammation and Immune Function
A number of molecules secreted by cells in the immune system serve as antimicrobials to fight infection. The most prevalent of these effectors include H2O2, xanthine, catalase, neuraminidase, and myeloperoxidase.
Learn More about Amplex® Red Assays for Inflammation and Immune Function
Neurotransmitters are fundamental to all neuronal function, from learning, memory and behavior to movement, sensation and autonomic function. Assays in this grouping are designed to measure the effect of drugs that alter their levels.
This section includes assays for three critical small molecules involved in normal cellular metabolism and important in disease states such as diabetes.
Learn More about Amplex® Red Assays for Metabolism
- Find out more about Molecular Probes® products and technologies.