Amplex® Red Enzyme Assays

Invitrogen offers validated solutions for enzyme activity assays using Molecular Probes® extremely versatile Amplex® Red reagent (and the second generation Amplex® UltraRed reagent).  This highly stable and sensitive fluorogenic substrate for horseradish peroxidase has been used to develop a variety of novel fluorogenic and chromogenic assays for enzymes that produce hydrogen peroxide. Additionally, these coupled  enzyme assays permit the ultrasensitive quantitation of a diverse assortment of analytes  including glucose, galactose, cholesterol, and hydrogen peroxide.


View All Amplex® Enzyme Assay Products

Amplex® Red Technology

How Amplex® Red Reagent Works

Amplex® Red reagent is a colorless substrate that reacts with hydrogen peroxide.

Amplex® UltraRed Reagent

 Our Amplex® UltraRed reagent improves upon the performance of the Amplex® Red reagent, offering brighter fluorescence and enhanced sensitivity.

Customize Your Amplex® Red Assay

We offer custom services with the Amplex® Red assays including bulk packaging, custom assay design and more.
Amplex® Red Assays Advantages
Amplex® Red and Amplex® UltraRed reagents over significant advantages over other oxidative enzyme assays. These include:

Increased sensitivity
  • Extended dynamic range and sensitivity compared to classic colorimetric oxidase assays
  • Fluorescence emission outside the range of compound autofluorescence

Quantitative enzyme activity detection

High Throughput compatibility
  • Antibody-free detection of analytes at pennies per well  
  • Scalable assay formats - custom packaging available

Ability to customize enzyme assays to fit your needs

Each kit comes complete with all the reagents needed to detect the enzyme and analytes in common biological samples, with standards included for precise quantitation.

Amplex® Red Assays for Multiple Research Areas

Cell Signaling and Lipids

Lipases and inorganic phosphates are critical in many signaling pathways, from mTOR to GPCRs where they are involved in disease states ranging from cardiovascular to inflammation and cancer.

Inflammation and Immune Function
A number of molecules secreted by cells in the immune system serve as antimicrobials to fight infection.  The most prevalent of these effectors include H2O2, xanthine, catalase, neuraminidase, and myeloperoxidase.


Learn More about Amplex® Red Assays for Inflammation and Immune Function
Neurobiology

Neurotransmitters are fundamental to all neuronal function, from learning, memory and behavior to movement, sensation and autonomic function.  Assays in this grouping are designed to measure the effect of drugs that alter their levels.


Learn More about Amplex® Red Assays for Neurobiology

Metabolism

This section includes assays for three critical small molecules involved in normal cellular  metabolism and  important in disease states such as diabetes.





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