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APEX™ Antibody Labeling Kits

APEX™ Antibody Lebeling Kits APEX™ Antibody Labeling Kits utilize a unique solid-phase labeling method that enables an efficient, convenient and reliable method for covalently attaching fluorescent labels to very small amounts of IgG antibody in the presence of any contaminants including stabilizing proteins and amine-containing buffers.

  • Reliability – Produces conjugates with stable, covalent attachment of superior fluorescent labels
  • Convenience – Requires only 10 –20 µg of IgG antibody per labeling reaction, regardless of any purity limitations
  • Complete - Includes everything required to perform 5 separate labelings

APEX™ Antibody Labeling Kits Products

FluorophoreEx/Em†Cat. no.
Emission Color*		Flow Cytometry
Imaging

Alexa Fluor® 488‡ 495/519A10468             ●
       ●

Alexa Fluor® 555555/565A10470
       ●
Alexa Fluor® 594590/617A10474
       ●
Alexa Fluor® 647
650/665A10475             ●
       ●
Oregon Green® 488‡ 496/524A10476             ●
       ●
Pacific Blue™ dye
410/455A10478             ●
       ●
* Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Fluorophore can also be used as a hapten for signal amplification with its corresponding anti-dye antibody.


How APEX™ Antibody Labeling Kits Work

The APEX™ Antibody Labeling Kits use a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX™ antibody labeling tip (Figure 1). Any contaminants, including stabilizing proteins or amine-containing buffers, are simply eluted through the tip. After applying the amine-reactive label, a fluorescent IgG conjugate is ready for use in any imaging or flow cytometry application in as little as 2.5 hours with very little hands-on time.
APEX™ Antibody Labeling Kits
Figure 1. APEX™ antibody labeling tip.
Each APEX™ Antibody Labeling Kit includes:

Material
Amount
Reactive fluorescent label
5 vials
APEX™ antibody labeling tips
5 each
APEX™ tip wash buffer
1.8 mL
Anhydrous DMSO
100 μL
APEX™ labeling buffer
100 μL
APEX™ neutralization buffer
100 μL
APEX™ elution buffer
1 ml


Product Information Sheet

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