May 23, 2013

Total Transcriptome Analysis on the Ion Torrent™ Platform

Dr. Triche received his AB in physics and biology from Cornell University, an MD and PhD degree from Tulane University Medical Center, and his pathology training at Washington University Medical Center. He was the PI on both NCI Director's Challenge and SPECS grants to develop diagnostic and prognostic/predictive RNA expression profiles for childhood cancer, and co-founded GenomeDx, a company using similar technology to predict clinical recurrence of prostate cancer after prostatectomy. He is the Chair Emeritus of the pathology department at Children's Hospital Los Angeles and serves as Director of the Center for Personalized Medicine at CHLA, as well as co-Director of the Institute for Molecular Medicine, a collaborative effort between Phoenix Children's Hospital and TGen.

RNA Sequencing for Biomarker Discovery

Audrey Papp is a research specialist in Pharmacology with expertise in Pharmacogenomics and Molecular Pathology. As Technical Director of the Program in Pharmacogenomics at The Ohio State University, she is instrumental in developing new methods and approaches geared towards discovery of functional genetic biomarkers. She has played a leading role in implementation of Next Generation Sequencing in the Pharmacogenomics Core Laboratory. The Pharmacogenomics Core Lab is currently using both the Ion PGM™, and Ion Proton™ deep sequencing platforms.

June 6, 2013

Performing Transcriptome Sequencing on the Ion Proton™ System

Sequencing is a powerful tool for looking at the complexity of the transcriptome. This webinar will discuss considerations for preparing your transcriptome sequencing experiments along with the use of Life Technologies solutions including the Ion Proton™ System. Both upfront sample preparation from Ambion and downstream data analysis solutions from Partek will be described.

Ion Reporter™ Software automates analysis, annotation, reporting, and archiving of Ion semiconductor sequencing data. Designed specifically for researchers performing routine assays, Ion Reporter™ Software helps you to quickly identify and easily interpret your DNA variants.

The webinar will describe how to use Avadis NGS for end-to-end RNA-Seq analysis workflow including alignment, quality control, analysis and biological contextualization.

Using Partek software, we will demonstrate how analysis of Ion Torrent RNA-Seq data can be accomplished with a few clicks of the mouse. We'll take you step-by-step through a sample set of Ion Torrent data, highlighting how to extract biological meaning from differentially expressed genes, examine alternative splicing and allele specific expression, and find novel transcripts. Supported with the robust statistical analysis, visualization tools, and advanced algorithms, Partek software easily turns raw sequence data into differential gene expression. No coding, just biology.

The webinar will describe how to use Avadis NGS for end-to-end RNA-Seq analysis workflow including alignment, quality control, analysis and biological contextualization.

Using Partek software, we will demonstrate how analysis of Ion Torrent RNA-Seq data can be accomplished with a few clicks of the mouse. We'll take you step-by-step through a sample set of Ion Torrent data, highlighting how to extract biological meaning from differentially expressed genes, examine alternative splicing and allele specific expression, and find novel transcripts. Supported with the robust statistical analysis, visualization tools, and advanced algorithms, Partek software easily turns raw sequence data into differential gene expression. No coding, just biology.

Join us as we reveal the critical steps to success in RNA sequencing. Starting from cells, we'll detail a workflow to obtain high quality RNA, perform rRNA depletion or enrich miRNA and build platform-specific libraries to enable next-generation sequencing runs. We will also detail the use of ERCC RNA Spike-In Control Mixes.

See the clear picture with Ion RNA-Seq and reveal the diversity of the transcriptome. RNA-Seq on the Ion PGM™ Sequencer has evolved. Register to join the webinar and learn how new protocols, kits, chips and software solutions are making Ion RNA-Seq even better.

Imagine how your lab can transform sequencing projects with a ready-to-use panel targeting "hot spot" regions in 46 genes, or with an extensive panel targeting all exons in 409 oncogenes and tumor suppressor genes. Or experience the power of sequencing a single 328-gene panel with 10,000 primer pairs that target variants in >700 inherited disorders including neuromuscular, cardiovascular, developmental, and metabolic diseases. This webinar is ideal for oncology or human disease researchers who want to transform their work.

You can now sequence hundreds of genes of your choice from just 10 ng of DNA. Create custom ultrahigh-multiplex PCR panels with Ion AmpliSeq™ Designer, a new free online assay design tool. The custom Ion AmpliSeq™ Panels contain up to 1,536 amplicons per tube, which require only 10 ng of input DNA for target selection. Get started today and go from DNA to variants in a single day.