Gibco® B-27® Supplements
 | For nearly 20 years, researchers have been publishing their experiments using B-27® supplements. The first commercially available serum-free supplement for neural cell culture, B-27® Serum Free Supplement was developed for the long-term culture of rat hippocampal and cortical neurons. Since its introduction, the publication history of B-27® supplements demonstrates that researchers are expanding the use of these supplements far beyond these original applications. |
The most referenced neural cell culture supplement
Available exclusively from Life Technologies, the B-27® family of supplements are the most referenced neural cell culture supplements. B-27® supplements have been cited in thousands of peer-reviewed publications spanning a variety of different cell types and used in the research of many different neurological disease models.
Which B-27® supplement is right for you?
To order the right B-27® supplement for you, click on the application/cell-type tab below and then click on the product name or catalog number to order.
All Gibco® neural cell culture reagents are optimized to work together as a complete media system. Need a complementary media or cells? Browse our neural cell culture product guide.
Standard Applications / Primary Neurons
| Application | Product name | Catalog No. |
|---|---|---|
| Growth and maintenance of neurons | B-27® Serum-Free Supplement | 17504044 |
Stem cells and Precursor cells
| Application | Product name | Catalog No. |
|---|---|---|
| Proliferation of stem cells | B-27® Supplement, minus vitamin A | 12587010 |
| Differentiation of stem cells | B-27® Supplement, XenoFree CTS™ | A14867-01 |
Specialty applications
| Application | Product name | Catalog No. |
|---|---|---|
| Studies where XenoFree components are required | B-27® Supplement, XenoFree CTS™ | A14867-01 |
| Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs | B-27® Supplement, minus AO (antioxidants) | 10889038 |
| Studies of insulin secretion or insulin receptors | B-27® Supplement, minus Insulin | 0050129SA |
| Electrophysiology studies | B-27® Electrophysiology Kit | A1413701 |
| Growth and expansion of retinal ganglion cells | B-27® Supplement, Custom | 0080085SA |
Table 1. Cell types and disease models cited in publications using Gibco® neural cell culture reagents
Primary cells | Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia nigral, sympathetic ganglia |
| Stem Cells | Induced pluripotent stem cell, neural precursor, neural stem cell, neuroepithelial |
| Glial Cells | Astrocyte, microglia, oligodendrocytes, radial glia, satellite, schwann |
| Disease Models | Alzheimer’s disease, amyotropyic lateral sclerosis (ALS), brain tumor, cerebral palsy, epilepsy, glioma, Huntington’s disease, multiple sclerosis, Parkinson’s disease, schizophrenia, spinal cord injury, stroke |
For research use only. Not for human or animal therapeutic or diagnostic use.
