Related Product Information |
Introduction
The Mark12™ Unstained Standard allows accurate molecular weight estimation of your protein sample over a wide molecular weight range.
The important features of the standard are listed below:
The important features of the standard are listed below:
- Consists of 12 protein bands in the range of 2.5-200 kDa
- Suitable for Tris-Glycine, Tricine, and NuPAGE® Novex Gels
- Visualized with Coomassie® or silver staining
${comergentUrl}
Protocol
The Mark12™ Unstained Standard is supplied ready-to-use. There is no need to heat or add reducing agent. For silver staining, dilute 1:20 in any SDS sample buffer.
Use loading volumes listed below to obtain the best results:
Use loading volumes listed below to obtain the best results:
| Gel | Volume |
|---|---|
| Mini-Gel (1.0 mm thick) | 5 µl |
| Mini-Gel (1.5 mm thick) | 7 µl |
| Standard Gel (1.0 mm thick) | 10 µl |
| Standard Gel (1.5 mm thick) | 15 µl |
Example
The approximate molecular weights of the protein bands in Mark12™ Unstained Standard are shown below. The molecular weights stated in the table below apply to the Tris-Glycine, Tricine, and NuPAGE® buffer systems.
Specifications
Contents: 1 ml of Mark12™ Unstained Standard
Applications: 200 of 5 μl each
Loading Buffer: Tris-HCl, Glycerol, SDS, Phenol Red, Coomassie Blue G-250
Storage: Store at 4°C
Stability: 6 months at 4°C
Applications: 200 of 5 μl each
Loading Buffer: Tris-HCl, Glycerol, SDS, Phenol Red, Coomassie Blue G-250
Storage: Store at 4°C
Stability: 6 months at 4°C
LT060
