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Introduction
The SeeBlue® Plus2 Pre-Stained Standard allows easy visualization of protein molecular weight ranges during electrophoresis and evaluation of western transfer efficiency. The important features of the standard are listed below:
- Consists of 10 pre-stained protein bands (8 blue and 2 contrasting colors) in the range of 4-250 kDa
- Suitable for Tris-Glycine, Tricine, and NuPAGE® Novex Gels
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Protocol
The SeeBlue® Plus2 Pre-Stained Standard is supplied ready-to-use. There is no need to heat or add reducing agent.
Use loading volumes listed below to obtain the best results:
Use loading volumes listed below to obtain the best results:
| Gel | Electrophoresis | Blotting |
|---|---|---|
| Mini-Gel (1.0 mm thick) | 10 µl | 3 µl |
| Mini-Gel (1.5 mm thick) | 20 µl | 5 µl |
| Standard Gel (1.0 mm thick) | 20 µl | 7 µl |
| Standard Gel (1.5 mm thick) | 30 µl | 10 µl |
Example
The apparent molecular weights of the protein bands in SeeBlue® Plus2 Pre-Stained Standard in several buffer systems is shown below. The protein bands have different mobilities in various SDS-PAGE buffer systems. For more information on this phenomenon, contact technical service or visit our Web site at www.invitrogen.com.
Specifications
Contents: 500 µl of SeeBlue® Plus2 Pre-Stained Standard
Loading Buffer: Tris-HCl, Formamide, SDS, Phenol Red
Storage: Store at 4°C
Stability: 4 months at 4°C
Loading Buffer: Tris-HCl, Formamide, SDS, Phenol Red
Storage: Store at 4°C
Stability: 4 months at 4°C
LT059
